- ￥4700
- GeneTex
- 美国
- GTX26161
- 2025年07月05日
- WB, ICC/IF, IHC-Fr, ELISA, RIA
- Rat
- Human, Mouse, Yeast, Drosophila, Arabidopsis thaliana, Ashbya, Asplenium nidus, Naegleria, Platyzoma, Seagrass (Cymodocea nodosa)
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Yeast tubulin
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Yeast, Drosophila, Arabidopsis thaliana, Ashbya, Asplenium nidus, Naegleria, Platyzoma, Seagrass (Cymodocea nodosa)
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Yeast
- 目录编号：
GTX26161
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rat
- 应用范围：
WB, ICC/IF, IHC-Fr, ELISA, RIA
- 浓度：
1.0 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
alpha Tubulin
- 抗体英文名：
alpha Tubulin antibody [YOL1/34]
- 抗体名：
alpha Tubulin 抗体 [YOL1/34]
- 规格：
100 μg
ICC/IF analysis of HeLa cells using GTX26161 alpha Tubulin antibody [YOL1/34].
Western blot analysis of CD107b on J774 cell lysate probed with Rat anti Mouse CD107b (GTX42498) at 1/500, 1/1000, 1/2000. Rat anti tubulin alpha (GTX26161) is included as a loading control.
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文献和实验Doi G et al., Nucleic Acids Res 2021 (PMID:33398345)
Zhang H et al., Methods Mol Biol 2004 (PMID:14970647)
Immunofluorescent Localization of Tubulin
Rabbit anti-tubulin (or other primary antibody to tubulin) FITC-labeled goat anti-rabbit (or secondary antibody to match the primary) Fluorescence Mounting Media Fluorescent Microscope equipped with 490 nm excitation filter
In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
the resulting pellets in 0.25 x V2 of cold PM buffer, incubate at on ice for 30 min and centrifuge again (steps 11-15). 18. The resulting cold supernatant is ~90% pure tubulin and is called twice-cycled tubulin
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