- ￥4700
- GeneTex
- 美国
- GTX26161
- 2025年07月05日
- WB, ICC/IF, IHC-Fr, ELISA, RIA
- Rat
- Human, Mouse, Yeast, Drosophila, Arabidopsis thaliana, Ashbya, Asplenium nidus, Naegleria, Platyzoma, Seagrass (Cymodocea nodosa)
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Yeast tubulin
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Yeast, Drosophila, Arabidopsis thaliana, Ashbya, Asplenium nidus, Naegleria, Platyzoma, Seagrass (Cymodocea nodosa)
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Yeast
- 目录编号：
GTX26161
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rat
- 应用范围：
WB, ICC/IF, IHC-Fr, ELISA, RIA
- 浓度：
1.0 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
alpha Tubulin
- 抗体英文名：
alpha Tubulin antibody [YOL1/34]
- 抗体名：
alpha Tubulin 抗体 [YOL1/34]
- 规格：
100 μg
ICC/IF analysis of HeLa cells using GTX26161 alpha Tubulin antibody [YOL1/34].
Western blot analysis of CD107b on J774 cell lysate probed with Rat anti Mouse CD107b (GTX42498) at 1/500, 1/1000, 1/2000. Rat anti tubulin alpha (GTX26161) is included as a loading control.
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文献和实验Doi G et al., Nucleic Acids Res 2021 (PMID:33398345)
Zhang H et al., Methods Mol Biol 2004 (PMID:14970647)
the resulting pellets in 0.25 x V2 of cold PM buffer, incubate at on ice for 30 min and centrifuge again (steps 11-15). 18. The resulting cold supernatant is ~90% pure tubulin and is called twice-cycled tubulin
LABELING ALPHA-ACTININ WITH IATR
with good stirring.The volume (ml)of borate buffer = mg IATR x 9 / (mg/ml)alpha-actinin. 4.Clarify IATR solution at 25,000 rpm,4℃,for 10 min in a 42.2Ti rotor. 5.Mix equal volumes of clarified dye and alpha-actinin in a test tube; remove
Immunofluorescent Localization of Tubulin
antibody to tubulin) FITC-labeled goat anti-rabbit (or secondary antibody to match the primary) Fluorescence Mounting Media Fluorescent Microscope equipped with 490 nm excitation filter and 515 nmbarrier filter Kodachrome film
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