IDH1 biotinylated mouse monocl
onal detection antibody, validated for ELISA and Luminex assays- ￥2700
- OriGene
- TA700198
- 2025年07月10日
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- 规格：
50 ug
predicted_size：null
Uniprot ID：O75874
description：IDH1 biotinylated mouse monoclonal detection antibody, validated for ELISA and Luminex assays
clonality：Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...)：Primary Antibodies
background：null
immunogen：Full length human recombinant protein of human IDH1 (NP_005887) produced in HEK293T cell.
recommended_dilution：ELISA 1:250-1:1000
predicted_size：null
buffer：Stored in PBS (pH 7.4) with 0.05% sodium azide, 10mg/ml BSA, 50% glycerol
host_species：Mouse
applications：ELISA
reactivities：Human, Mouse, Rat
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文献和实验E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
is an indirect expression of the monoclonal antibody amount in the sample.Methods1 Obtain a 96-well plate and rinse 3x with PBS.2 Add 50 µl of a 1 mg/ml of antigen (venom, serum, or fraction), incubate for 30 min at 37°C.3 Rinse with PBS 3x.4 Add 5% powdered milk
RAT/MOUSE GROWTH HORMONE ELISA KIT
after each wash to remove residual buffer. 8) Add 100 µL Detection Antibody to all wells. Cover the plate with plate sealer and incubate at room temperature for 1 hour on an orbital microtiter plate shaker set to rotate at moderate speed, approximately 400
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