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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
The antiserum was produced against a chemically synthesized peptide derived from the human BID fragment (p15).
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX25498
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
Recognizes the human C-terminal 15 kDa fragment of BID (p15).
- 抗体英文名:
Bid (p15-specific ) antibody
- 抗体名:
Bid (p15-specific ) 抗体
- 规格:
50 μl
WB analysis of Jurkat cells without added BID (Lanes 1 and 2), with caspase-8 cleaved recombinant human BID (Lane 3), or with caspase-8 cleaved recombinant mouse BID (Lane 4) using either a human BID full length antibody (lane 1) or GTX25498 Bid (p15-specific ) antibody (lanes 2-4). The data show that the human BID (p15) antibody recognizes only the human 15 kDa BID fragment, demonstrating the specificity of the antibody.
ICC/IF analysis of A549 cells using GTX25498 Bid (p15-specific ) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% PFA
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 1% BSA incubated for 3 hours at room temperature
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文献和实验Li Y et al., Mol Cell Biochem 2009 (PMID:19521670)
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