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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Integrin b1. This sequence is conserved among many species including mouse, rat and chicken.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Mouse, Chicken
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX25185
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
Integrin beta 1 / CD29 (phospho Ser785)
- 抗体英文名:
Integrin beta 1 / CD29 (phospho Ser785) antibody
- 抗体名:
Integrin beta 1 / CD29 (phospho Ser785) 抗体
- 规格:
50 μl
WB analysis of Integrin beta 1-deficient fibroblasts expressing wild type (Lane 1-5, 7) or serine-785-alanine mutant (S785A: Lane 6, 8) chicken Integrin beta 1 using GTX25185 Integrin beta 1 / CD29 (phospho Ser785) antibody. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
3.1 and 3.6 ). This will provide a “specific activity” measurement relative to total protein in the cell, and is themost accurate measure of total GSK3 activity, or2. Immunoblot using antibodies to phospho-Ser21 (GSK3a)or phospho-Ser9 (GSK3b) (see Sections 3.1 –
[分享] Strip Antibodies and Re-use Protein blots membrane
off twice with TBS-T I've probed the same blots for different antibodies, tubulin after using a phospho antibody. Seven: 0.5M Acetic acid 0.5M NaCl Eight: After your first probing,Keep the PVDF membrane wet. 1. Wash it in ddWater for 10 min 2. Wash
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