- ￥5600
- GeneTex
- 美国
- GTX24774
- 2025年07月13日
- WB, ICC/IF, FACS
- Rabbit
- Human, Mouse, Rat, Rabbit
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- 详细信息
- 技术资料
- 免疫原：
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human eIF4E that contains serine 209. The sequence is conserved in mouse, rat and rabbit.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Rabbit
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX24774
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, FACS
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
eIF4E (phospho Ser209)
- 抗体英文名：
eIF4E (phospho Ser209) antibody
- 抗体名：
eIF4E (phospho Ser209) 抗体
- 规格：
50 μl
ICC/IF analysis of U-87 MG cells using GTX24774 eIF4E (phospho Ser209) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% PFA
Permeabilization : 0.1% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature
WB analysis of whole cell extracts (20 μg lysate) of HeLa (lane 1), Serum Starved HeLa (lane 2), serum starved for overnight followed by Serum Released (lane 3), HEK-293 (lane 4), treated for 30 minutes with 25 μg/mL of Anisomycin (lane 5), L6 (lane 6) and L6 treated for 10 minutes with 200 ng/ml of Insulin (lane 7) using GTX24774 eIF4E (phospho Ser209) antibody.
Dilution : 1:500-1:2000
WB (peptide competition) analysis of HeLa cells using GTX24774 eIF4E (phospho Ser209) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 2), a generic phosphoserine-containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.
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