Phospho-eIF4E (Ser209) Antibod

9741:

Western Blotting

Phospho-eIF4E (Ser209) Antibody detects endogenous levels of eIF4E only when phosphorylated at serine 209.

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser209 of human eIF4E. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or treated with serum, PD98059 or Dexamethasone, using Phospho-eIF4E (Ser209) Antibody (upper) or eIF4E Antibody #9742 (lower).

Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure to mediate the initiation of translation (1,2). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (2). eIF4B is thought to assist the eIF4F complex in translation initiation. Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 phosphorylates eIF4E at Ser209 in vivo (3,4). Two Erk and p38 MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for Mnk1 kinase activity (3). The carboxy-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148, and Ser1192 (5). Phosphorylation at these sites is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin.

1. Sonenberg, N. et al. (1978) Proc. Natl. Acad. Sci. USA 75, 4843-4847.
2. Gingras, A.C. et al. (1999) Annu. Rev. Biochem. 68, 913-963.
3. Waskiewicz, A. et al. (1999) Mol. Cell. Biol. 19, 1871-1880.
4. Pyronnet, S. et al. (1999) EMBO J. 18, 270-279.
5. Raught, B. et al. (2000) EMBO J. 19, 434-444.

• He, Y. et al. (2001) Regulation of mRNA translation and cellular signaling by hepatitis C virus nonstructural protein NS5A. J. Virol. 75, 5090-5098. Applications: Western Blot
• Banerjee, S. et al. (2002) Murine coronavirus replication-induced p38 mitogen-activated protein kinase activation promotes interleukin-6 production and virus replication in cultured cells. J. Virol. 76, 5937-4948. Applications: Western Blotting
• Lindemann, S. et al. (2001) Integrins regulate the intracellular distribution of eukaryotic initiation factor 4E in platelets: A checkpoint for translational control. J. Biol. Chem. 276, 33947-33951. Applications: Western Blotting
• Pyronnet, S. et al. (2001) Suppression of cap-dependent translation in mitosis. Genes Dev. 15, 2083-2093. Applications: Western Blotting
• Ma, D. et al. (2009) J Cell Physiol 219, 31-44. Applications: Western Blotting
• RamachandraRao, S.P. et al. (2009) J Am Soc Nephrol 20, 1765-75. Applications: Western Blotting
• Wu, M. et al. (2010) Rejuvenation Res 13, 571-9. Applications: Western Blotting

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For Research Use Only. Not For Use In Diagnostic Procedures.