- ￥5600
- GeneTex
- 美国
- GTX24736
- 2025年07月16日
- WB, IHC-P
- Rabbit
- Human, Mouse
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- 详细信息
- 技术资料
- 免疫原：
Chemically synthesized phosphopeptide derived from a region of human ATF2 that contains threonine 71
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX24736
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IHC-P
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
ATF2 (phospho Thr71)
- 抗体英文名：
ATF2 (phospho Thr71) antibody
- 抗体名：
ATF2 (phospho Thr71) 抗体
- 规格：
50 μl
IHC-P analysis of human breast carcinoma tissue using GTX24736 ATF2 (phospho Thr71) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20
WB analysis of whole cell extracts (30 μg lysate) of U-87 MG (Lane 1), U-87 MG treated with 25 μg/mL anisomycin for 30 minutes (Lane 2), K562 (Lane 3), K562 treated with 25 μg/mL anisomycin for 30 minutes (Lane 4) using GTX24736 ATF2 (phospho Thr71) antibody.
Dilution : 1-2 μg/ml
IHC-P analysis of mouse brain tissue using GTX24736 ATF2 (phospho Thr71) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20
WB (peptide competition) analysis of Jurkat cells treated with 10 μg/mL anisomycin for 60 minutes using GTX24736 ATF2 (phospho Thr71) antibody prior incubated with the phosphopeptide immunogen (Lane 2), the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), or a generic phosphothreonine-containing peptide (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.
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