- ￥5600
- GeneTex
- 美国
- GTX24736
- 2025年07月16日
- WB, IHC-P
- Rabbit
- Human, Mouse
企业认证
相关产品推荐更多 >
![Plasma Kallikrein 1B antibody [13G11]](https://img1.dxycdn.com/2023/0711/451/6106825198874858761-14.jpg!wh200)
![PIST antibody [C3], C-term](https://img1.dxycdn.com/2023/0516/045/6925717576889828561-14.jpg!wh200)
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Chemically synthesized phosphopeptide derived from a region of human ATF2 that contains threonine 71
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX24736
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IHC-P
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
ATF2 (phospho Thr71)
- 抗体英文名：
ATF2 (phospho Thr71) antibody
- 抗体名：
ATF2 (phospho Thr71) 抗体
- 规格：
50 μl
IHC-P analysis of human breast carcinoma tissue using GTX24736 ATF2 (phospho Thr71) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20
WB analysis of whole cell extracts (30 μg lysate) of U-87 MG (Lane 1), U-87 MG treated with 25 μg/mL anisomycin for 30 minutes (Lane 2), K562 (Lane 3), K562 treated with 25 μg/mL anisomycin for 30 minutes (Lane 4) using GTX24736 ATF2 (phospho Thr71) antibody.
Dilution : 1-2 μg/ml
IHC-P analysis of mouse brain tissue using GTX24736 ATF2 (phospho Thr71) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20
WB (peptide competition) analysis of Jurkat cells treated with 10 μg/mL anisomycin for 60 minutes using GTX24736 ATF2 (phospho Thr71) antibody prior incubated with the phosphopeptide immunogen (Lane 2), the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), or a generic phosphothreonine-containing peptide (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads
neutralization of phospho-specific antibodies with phospho-peptides immobilized on magnetic beads. This technique allows for sequestration of antibody–peptide complex from the incubation solution, minimizing the risk of formation of unblocked antibodies capable
技术资料暂无技术资料 索取技术资料
