Phosphotyrosine antibody [13F9]

Phosphotyrosine antibody [13F9

]
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  • ¥5300
  • GeneTex已认证
  • 美国
  • GTX22287
  • 2025年07月12日
  • WB, ELISA
  • Mouse
  • Species independent
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    • 详细信息
    • 技术资料
    • 免疫原

      Phosphotyrosine conjugated KLH.

    • 亚型

      IgG1

    • 形态

      Liquid

    • 保存条件

      Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

    • 克隆性

      Monoclonal

    • 标记物

      Unconjugated

    • 适应物种

      Species independent

    • 保质期

      12 months from the shipping date of the product. 

    • 目录编号

      GTX22287

    • 级别

      Primary Antibodies

    • 库存

      Available

    • 供应商

      GeneTex

    • 宿主

      Mouse

    • 应用范围

      WB, ELISA

    • 浓度

      Batch dependent (Please refer to the vial label for the specific concentration.)

    • 靶点

      Reactivity is specific for phosphotyrosine and minimal cross reactivity is observed against phosphoserine or phosphothreonine.

    • 抗体英文名

      Phosphotyrosine antibody [13F9]

    • 抗体名

      Phosphotyrosine 抗体 [13F9]

    • 规格

      50 μl

    Phosphotyrosine antibody [13F9

    Western Blot of Mouse anti-phosphotyrosine antibody (clone 13F9) (GTX22287). Lane 1: phosphorylated EGFR in an A431 cell lysate. Lane 2: total protein in the unstimulated A431 cells lysate after coommassie staining. Load: 10 μg per lane. Primary antibody: phosphotyrosine antibody at 1:1000 for 1 hr at RT. Secondary antibody:Goat-a-Mouse IgG [H&L] secondary antibody diluted 1:2,500 for 30' at room temperature. Block: 5% BLOTTO overnight at 4ºC.

    Phosphotyrosine antibody [13F9

    ELISA of Mouse anti-phosphotyrosine antibody (GTX22287). Antigen: BSA conjugates of pT, pY and pS. Coating amount: 0.1 μg per well. Primary antibody: Phosphotyrosine antibody at 1:1000. Dilution series (X-axis): 2-fold. Secondary antibody: HRP conjugated Gt-a-Mouse IgG H&L secondary antibody at 1:20,000. Substrate: TMB

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