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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Phosphotyrosine conjugated KLH.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Species independent
- 保质期:
12 months from the shipping date of the product.
- 目录编号:
GTX22287
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ELISA
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
Reactivity is specific for phosphotyrosine and minimal cross reactivity is observed against phosphoserine or phosphothreonine.
- 抗体英文名:
Phosphotyrosine antibody [13F9]
- 抗体名:
Phosphotyrosine 抗体 [13F9]
- 规格:
50 μl
Western Blot of Mouse anti-phosphotyrosine antibody (clone 13F9) (GTX22287). Lane 1: phosphorylated EGFR in an A431 cell lysate. Lane 2: total protein in the unstimulated A431 cells lysate after coommassie staining. Load: 10 μg per lane. Primary antibody: phosphotyrosine antibody at 1:1000 for 1 hr at RT. Secondary antibody:Goat-a-Mouse IgG [H&L] secondary antibody diluted 1:2,500 for 30' at room temperature. Block: 5% BLOTTO overnight at 4ºC.
ELISA of Mouse anti-phosphotyrosine antibody (GTX22287). Antigen: BSA conjugates of pT, pY and pS. Coating amount: 0.1 μg per well. Primary antibody: Phosphotyrosine antibody at 1:1000. Dilution series (X-axis): 2-fold. Secondary antibody: HRP conjugated Gt-a-Mouse IgG H&L secondary antibody at 1:20,000. Substrate: TMB
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文献和实验Enrichment of Phosphotyrosine Proteome of Human Platelets by Immunoprecipitation
Proteomics offers the opportunity to comprehensively investigate the anucleate platelet. Here, we present a detailed procedure for enrichment by immunoprecipitation, using the monoclonal antibody 4G10, of the dynamic phosphotyrosine proteome
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