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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Peptide corresponding to aa 505-521 at C-terminus of human FLICE2 (accession no. AAD28402).
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX22012
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, ELISA
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
This antibody recognizes full-length human caspase-10 (59kDa). Since the sequences at C- termini of FLICE2 and Mch4 are different, this antibody recognizes only the FLICE2 form of caspase-10.
- 抗体英文名:
Caspase 10 antibody
- 抗体名:
Caspase 10 抗体
- 规格:
100 μg
ICC/IF analysis of HeLa cells using GTX22012 Caspase 10 antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 20 μg/ml
Western blot analysis of Caspase-10 in HeLa (H), Jurkat (J), A431 (A), K562 (K) whole cell lysates with Caspase-10 antibody (GTX22012) at 1μg/ml.
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文献和实验Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit
的Caspase-3及对底物多聚(ADP-核糖)聚合酶[poly(ADP-ribose)polymerase,PARP]等的裂解。方法:收集细胞→PBS洗涤→抽提细胞裂解液→蛋白定量→SDS-PAGE电泳→硝酸纤维素膜或PVDF膜转移→5%脱脂奶粉封闭,室温1.5~2h或4°C过夜→Caspase-3多抗或单抗室温反应1~2h或4°C过夜→TBS-T(含0.05% Tween 20的TBS)洗3次,5~10min/次→HRP-标记的羊抗鼠IgG或AP标记的羊抗鼠IgG室温反应1~2h→ TBS-T洗3次
浓度的值差不多。 问题 1. 我的control孔的相对活化程度也有2,这是不是说明细胞状态不好呢。我的细胞密度是4*10^6,6孔板2mL 2. 我这个结果可信不?还是可能因为我们的化合物还有其他非基于caspase-3的凋亡途径呢? 求解答。谢谢、 筱七 您好,请问您做出来了吗?我也是用凯基的,测出来的值很小很小。我用六孔板养的细胞,测出来的蛋白量远超过了200ug,所以当时取了200ug测的,但是值好小呀。问凯基
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