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400 ul
predicted_size：null
Uniprot ID
description：Hantavirus (Hantaan Nucleocapsid) mouse monoclonal antibody, clone B5D9, Supernatant
clonality：Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...)：Primary Antibodies
background：null
immunogen：Recombinant nucleocapsid protein of Hantaan 76-118 strain.
recommended_dilution：Western blotting. Immunofluorescence. Immunohistochemistry on Frozen Sections: 1/50. Use PBS, pH 7.2, with 0.5% BSA and 0.09% Sodium Azide as dilution buffer. Successful use in ELISA was reported by some researchers. As this detection method has not been verified by Acris Antibodies, the application was deleted from the database. This does not necessarily exclude the use in such procedure.
predicted_size：null
buffer：State: Supernatant; State: Liquid Hybridoma Culture Supernatant; Preservative: 0.09% Sodium Azide
host_species：Mouse
applications：IF, IHC, WB
reactivities：Hantaan Virus
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production
Monoclonal Antibody Production Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform
Rapid Isotyping of Mouse Monoclonal Antibodies
, isotyping is useful to control lot to lot consistency, detect contamination by another antibody, and identify hybridoma switch variants (see Notes 2 –8 for further comment on applications). Table 1 Isotype-Dependent Properties of Monoclonal
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