![M13 antibody [E1] (Biotin)产品图](https://img1.dxycdn.com/2023/0711/451/6106825198874858761-14.jpg)
- ￥5000
- GeneTex
- 美国
- GTX17269
- 2025年07月11日
- WB, FACS, ELISA, IHC
- Mouse
- Bacteriophage M13
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- 文献和实验
- 技术资料
- 免疫原：
Full length M13 phage coat protein; Exact epitope not determined.
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Biotin
- 适应物种：
Bacteriophage M13
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX17269
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, FACS, ELISA, IHC
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
M13 antibody [E1] (GTX17269) is specific for gp3 protein.
- 抗体英文名：
M13 antibody [E1] (Biotin)
- 抗体名：
M13 抗体 [E1] (Biotin)
- 规格：
50 μg
Data respresents absorbancy readings for A10B phage on rabbit IgG (A10B/IgG), A10B phage on BSA (A10B/BSA), streptavidin on rabbit IgG (SA/IgG) and streptavidin on BSA (SA/BSA) for each dilution of biotinylated anti-M13 monoclonal antibody.
Colo320 cell line stained with a cell-line specific M13 phage antibody (filled profile), compared to an irrelevant phage antibody (open profile). Cells were incubated overnight with 1012 phage particles, then detected with 10ug/ml anti-M13 antibody followed by rabbit anti-mouse IgG:FITC
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文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room
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