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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A fragment of the human Nestin protein corresponding to residues 1464-1614. [UniProt P48681]
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Hamster
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX30671
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IHC-P, IHC-Fr, FACS, MS
- 浓度:
2.4 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
We do not recommend use of this product for Rabbit,Rat.
- 抗体英文名:
Nestin antibody [10C2]
- 抗体名:
Nestin 抗体 [10C2]
- 规格:
100 μl
FACS (Intracellular staining) analysis of 1 x 10⁶ CHO (A) and HEK293 (B) cells using GTX30671 Nestin antibody [10C2].
Blue : Primary antibody
Orange : isotype control
Dilution : 1 μg/10⁶ cells
ICC/IF analysis of F9 cells using GTX30671 Nestin antibody [10C2].
Green : primary antibody(left) and isotype control(right)
Blue : DAPI
Dilution : 1:50
Permibilization : 0.1% Triton X-100
IHC-P analysis of human kidney tissue using GTX30671 Nestin antibody [10C2].
Dilution : 1:500
WB analysis of (1) rat brain tissue, and (2) human CNS progenitor cell lysate using GTX30671 Nestin antibody [10C2]. The antibody did not react with Rat, whereas, in lysate from human samples, it generated a doublet band representing the phosphorylated and the non-phosphorylated forms of Nestin protein.
Loading : 5μg
Dilution : 1:1000
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文献和实验Yang JL et al., Neural Regen Res 2023 (PMID:36571357)
Ana G Pi?a-Medina et al., Life Sci 2020 (PMID:32165211)
Lu P et al., J Clin Invest 2017 (PMID:28825600)
Garcia-Castro IL et al., PLoS One 2015 (PMID:26720151)
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