- ￥6700
- GeneTex
- 美国
- GTX30671
- 2025年07月12日
- WB, ICC/IF, IHC-P, IHC-Fr, FACS, MS
- Mouse
- Human, Mouse, Hamster
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A fragment of the human Nestin protein corresponding to residues 1464-1614. [UniProt P48681]
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Hamster
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX30671
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr, FACS, MS
- 浓度：
2.4 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
We do not recommend use of this product for Rabbit,Rat.
- 抗体英文名：
Nestin antibody [10C2]
- 抗体名：
Nestin 抗体 [10C2]
- 规格：
100 μl
FACS (Intracellular staining) analysis of 1 x 10⁶ CHO (A) and HEK293 (B) cells using GTX30671 Nestin antibody [10C2].
Blue : Primary antibody
Orange : isotype control
Dilution : 1 μg/10⁶ cells
ICC/IF analysis of F9 cells using GTX30671 Nestin antibody [10C2].
Green : primary antibody(left) and isotype control(right)
Blue : DAPI
Dilution : 1:50
Permibilization : 0.1% Triton X-100
IHC-P analysis of human kidney tissue using GTX30671 Nestin antibody [10C2].
Dilution : 1:500
WB analysis of (1) rat brain tissue, and (2) human CNS progenitor cell lysate using GTX30671 Nestin antibody [10C2]. The antibody did not react with Rat, whereas, in lysate from human samples, it generated a doublet band representing the phosphorylated and the non-phosphorylated forms of Nestin protein.
Loading : 5μg
Dilution : 1:1000
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文献和实验Yang JL et al., Neural Regen Res 2023 (PMID:36571357)
Ana G Pi?a-Medina et al., Life Sci 2020 (PMID:32165211)
Lu P et al., J Clin Invest 2017 (PMID:28825600)
Garcia-Castro IL et al., PLoS One 2015 (PMID:26720151)
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Analysis of mRNA Populations from Single Live and Fixed Cells of the Central Nervous System
amplification of individual mRNA species, differential display of subpopulations of the mRNA, or to make cDNA libraries. Nestin antibody (AN129, diluted 1:1000 for these studies) was generously provided by Dr. J. Trojanowski, Univ. of Pennsylvania
or stressed conditions can be studied using a monolayer cell?culture system of the nestin?expressing NSPC lineage to analyze proliferation, survival, and differentiation. Here, a protocol for the expansion of NSPCs for studies aimed at understanding
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