pCXSN植物表达质粒

基本信息

启动子:	CaMV 35S promoter
复制子:	pUC ori，pVS1 oriV
终止子:	NOS terminator
质粒分类:	植物系列质粒；植物表达质粒；其它系列质粒
质粒大小:	10802bp
质粒标签:	ccdB致死基因
原核抗性:	卡那霉素Kan
筛选标记:	潮霉素Hyg
克隆菌株:	Stbl3等大肠杆菌
培养条件:	37℃，有氧，LB
表达宿主:	植物
诱导方式:	无需诱导，组成型表达
3'测序引物:	NOS-ter：cccatctcataaataacgtc

质粒简介

         pCXSN质粒是一个植物过表达载体，用Xcm I 酶切后变成T载体，连入基因后可直接转化。

质粒图谱

质粒序列

LOCUS       Exported               10802 bp ds-DNA     circular SYN 25-AUG-2017
DEFINITION  synthetic circular DNA
KEYWORDS    pCXSN
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10802)
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2017 from Plasmid
FEATURES             Location/Qualifiers
     source          1..10802
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      8..260
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     119..138
                     /label=NOS-ter
     CDS             627..932
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     promoter        complement(1002..1892)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    2126..2150
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3449..4078
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4512..5579
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      5645..5839
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6183..6323
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6509..7097)
                     /direction=LEFT
                     /label=pUC ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7184..7978)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8403..8427
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    8505..8679
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8719..9744)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(9811..10488)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 gaattccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
       61 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
      121 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
      181 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
      241 tgccaaatgt ttgaacgatc ggggaaattc gctagtggat ccccaatact tgtatggggc
      301 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata
      361 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta
      421 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc
      481 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
      541 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
      601 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
      661 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga
      721 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
      781 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
      841 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
      901 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
      961 ctgcaggtcg acccatacaa gtattgggga tccccctcga gtatcgttcg taaatggtga
     1021 aaattttcag aaaattgctt ttgctttaaa agaaatgatt taaattgctg caatagaagt
     1081 agaatgcttg attgcttgag attcgtttgt tttgtatatg ttgtgttgag gtcgaggtcc
     1141 tctccaaatg aaatgaactt ccttatatag aggaagggtc ttgcgaagga tagtgggatt
     1201 gtgcgtcatc ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg
     1261 ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac
     1321 tgtcggcaga ggcatcttca acgatggcct ttcctttatc gcaatgatgg catttgtagg
     1381 agccaccttc cttttccact atcttcacaa taaagtgaca gatagctggg caatggaatc
     1441 cgaggaggtt tccggatatt accctttgtt gaaaagtctc aattgccctt tggtcttctg
     1501 agactgtatc tttgatattt ttggagtaga caagtgtgtc gtgctccacc atgttatcac
     1561 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
     1621 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttcaacg atggcctttc
     1681 ctttatcgca atgatggcat ttgtaggagc caccttcctt ttccactatc ttcacaataa
     1741 agtgacagat agctgggcaa tggaatccga ggaggtttcc ggatattacc ctttgttgaa
     1801 aagtctcaat tgccctttgg tcttctgaga ctgtatcttt gatatttttg gagtagacaa
     1861 gtgtgtcgtg ctccaccatg ttgacctgca ggcatgcaag cttggcactg gccgtcgttt
     1921 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     1981 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     2041 tgcgcagcct gaatggcgaa tgctagagca gcttgagctt ggatcagatt gtcgtttccc
     2101 gccttcagtt taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa
     2161 agagcgttta ttagaataac ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc
     2221 atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc
     2281 cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga
     2341 catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt
     2401 tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta
     2461 cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga
     2521 ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc
     2581 cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct
     2641 agccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac
     2701 ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag
     2761 ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt
     2821 gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag
     2881 gcccgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc
     2941 cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg
     3001 catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc
     3061 aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc
     3121 gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt
     3181 ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc
     3241 ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc
     3301 tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt
     3361 gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag
     3421 taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg
     3481 cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc
     3541 cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg
     3601 ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa
     3661 ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc
     3721 tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga
     3781 catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg
     3841 aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga
     3901 ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg
     3961 cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg
     4021 cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt
     4081 taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc
     4141 gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg
     4201 gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa
     4261 ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc
     4321 aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga
     4381 acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg
     4441 cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga
     4501 atcggcgtga cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg
     4561 acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag
     4621 cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac
     4681 cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt
     4741 ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg
     4801 ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc
     4861 cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg
     4921 acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga
     4981 agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc
     5041 ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca
     5101 ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat
     5161 ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg
     5221 agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc
     5281 cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc
     5341 tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga
     5401 tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc
     5461 tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc
     5521 cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat
     5581 gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct
     5641 ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt
     5701 acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa
     5761 aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa
     5821 cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc
     5881 ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg
     5941 ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg
     6001 cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt
     6061 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     6121 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     6181 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     6241 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     6301 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6361 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6421 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6481 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6541 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6601 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6661 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6721 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6781 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6841 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6901 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     6961 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7021 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7081 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7141 acgaaaactc acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca
     7201 gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata
     7261 gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt
     7321 cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat
     7381 ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg
     7441 gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt
     7501 cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta
     7561 agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc
     7621 tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt
     7681 ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt
     7741 caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt
     7801 cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt
     7861 tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta
     7921 cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt ttagccattt
     7981 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa
     8041 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt
     8101 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc
     8161 gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga
     8221 gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac
     8281 atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg
     8341 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct
     8401 ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
     8461 cggacgtttt taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta
     8521 ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat
     8581 acatactaag ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaatt
     8641 cccttatctg ggaactactc acacattatt atggagaaac tcgagcttgt cgatcgacag
     8701 atccggtcgg catctactct atttctttgc cctcggacga gtgctggggc gtcggtttcc
     8761 actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat
     8821 ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc
     8881 ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat
     8941 gcggagcata tacgcccgga gtcgtggcga tcctgcaagc tccggatgcc tccgctcgaa
     9001 gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac
     9061 ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc
     9121 attgtccgtc aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg
     9181 gcagtcctcg gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt
     9241 gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc
     9301 acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg
     9361 gctaagatcg gccgcagcga tcgcatccat agcctccgcg accggttgta gaacagcggg
     9421 cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg agatgcaata
     9481 ggtcaggctc tcgctaaact ccccaatgtc aagcacttcc ggaatcggga gcgcggccga
     9541 tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg
     9601 caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga
     9661 gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt
     9721 cgcggtgagt tcaggctttt tcatatctca ttgccccccg gatctgcgaa agctcgagag
     9781 agatagattt gtagagagag actggtgatt tcagcgtgtc ctctccaaat gaaatgaact
     9841 tccttatata gaggaaggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca
     9901 gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc
     9961 acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga
    10021 acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact
    10081 gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt
    10141 accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc
    10201 ttggagtaga cgagagtgtc gtgctccacc atgttatcac atcaatccac ttgctttgaa
    10261 gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg
    10321 ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat
    10381 ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa
    10441 tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg
    10501 tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg
    10561 ttggcaagct gctctagcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    10621 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    10681 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    10741 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
    10801 ac