pCXSN-Myc植物表达质粒

基本信息

启动子:	35S promoter
终止子:	NOS terminator
质粒大小:	10835bp
质粒标签:	N-Myc
原核抗性:	卡那霉素Kan
筛选标记:	潮霉素Hyg
克隆菌株:	大肠杆菌DB3.1
培养条件:	37℃，有氧，LB

质粒简介

            pCXSN-Myc质粒是一个植物过表达质粒，35S启动子驱动Myc标签和目的基因融合表达，在ccdb致死基因的作用下，克隆基因变得更加容易，阳性率高。

质粒图谱

质粒序列

LOCUS       Exported               10835 bp ds-DNA     circular SYN 30-OCT-2017
DEFINITION  synthetic circular DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10835)
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10835
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      7..270
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             627..932
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(988..1017)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(1035..1925)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    2159..2183
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3482..4111
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4545..5612
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      5678..5872
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6216..6356
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6542..7130)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7217..8011)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8436..8460
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    8538..8712
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8752..9777)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(9844..10521)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 gaattccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
       61 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
      121 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
      181 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
      241 tgccaaatgt ttgaacgatc ggggaaattc gctagtggat ccccaatact tgtatggggc
      301 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata
      361 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta
      421 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc
      481 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
      541 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
      601 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
      661 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga
      721 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
      781 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
      841 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
      901 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
      961 ctgcaggtcg acccatacaa gtattggaag atcttcttca gaaatcaact tttgttccat
     1021 ggatccccct cgagtatcgt tcgtaaatgg tgaaaatttt cagaaaattg cttttgcttt
     1081 aaaagaaatg atttaaattg ctgcaataga agtagaatgc ttgattgctt gagattcgtt
     1141 tgttttgtat atgttgtgtt gaggtcgagg tcctctccaa atgaaatgaa cttccttata
     1201 tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga
     1261 tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc
     1321 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tcaacgatgg
     1381 cctttccttt atcgcaatga tggcatttgt aggagccacc ttccttttcc actatcttca
     1441 caataaagtg acagatagct gggcaatgga atccgaggag gtttccggat attacccttt
     1501 gttgaaaagt ctcaattgcc ctttggtctt ctgagactgt atctttgata tttttggagt
     1561 agacaagtgt gtcgtgctcc accatgttat cacatcaatc cacttgcttt gaagacgtgg
     1621 ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac
     1681 tgtcggcaga ggcatcttca acgatggcct ttcctttatc gcaatgatgg catttgtagg
     1741 agccaccttc cttttccact atcttcacaa taaagtgaca gatagctggg caatggaatc
     1801 cgaggaggtt tccggatatt accctttgtt gaaaagtctc aattgccctt tggtcttctg
     1861 agactgtatc tttgatattt ttggagtaga caagtgtgtc gtgctccacc atgttgacct
     1921 gcaggcatgc aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     1981 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     2041 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga
     2101 gcagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg
     2161 acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat aacggatatt
     2221 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
     2281 ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt
     2341 ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg
     2401 acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa
     2461 gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg
     2521 cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga
     2581 actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga
     2641 ccgcccggag ctggccagga tgcttgacca cctagccctg gcgacgttgt gacagtgacc
     2701 aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag
     2761 gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc
     2821 ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc
     2881 gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc
     2941 cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc
     3001 accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt
     3061 gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac
     3121 gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca
     3181 tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg
     3241 agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc
     3301 atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg
     3361 ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc
     3421 gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg
     3481 catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac
     3541 ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc
     3601 ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg
     3661 catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt
     3721 gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt
     3781 cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga
     3841 gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc
     3901 gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga
     3961 gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc
     4021 cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct
     4081 ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa
     4141 agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg
     4201 gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat
     4261 cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct
     4321 gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt
     4381 agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg
     4441 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc
     4501 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg
     4561 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc
     4621 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg
     4681 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta
     4741 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg
     4801 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc
     4861 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag
     4921 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc
     4981 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc
     5041 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag
     5101 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga
     5161 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct
     5221 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt
     5281 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt
     5341 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag
     5401 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag
     5461 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt
     5521 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc
     5581 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg
     5641 ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga
     5701 acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg
     5761 ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct
     5821 aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg
     5881 gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac
     5941 gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac
     6001 ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc
     6061 cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     6121 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     6181 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     6241 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     6301 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
     6361 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6421 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6481 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6541 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6601 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6661 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6721 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6781 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6841 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6901 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6961 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     7021 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7081 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7141 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7201 tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc
     7261 aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat
     7321 cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc
     7381 tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca
     7441 ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca
     7501 gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca
     7561 gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg
     7621 gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa
     7681 tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac
     7741 tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca
     7801 gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt
     7861 tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata
     7921 ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt
     7981 tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt ttctacagta
     8041 tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc
     8101 attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta
     8161 taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg
     8221 tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc
     8281 ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg
     8341 gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg
     8401 ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac
     8461 aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac
     8521 gccgaattaa ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa
     8581 ttttattgat agaagtattt tacaaataca aatacatact aagggtttct tatatgctca
     8641 acacatgagc gaaaccctat aggaacccta attcccttat ctgggaacta ctcacacatt
     8701 attatggaga aactcgagct tgtcgatcga cagatccggt cggcatctac tctatttctt
     8761 tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc
     8821 atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc
     8881 ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc
     8941 aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagtcgtgg
     9001 cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc
     9061 caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc
     9121 ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc
     9181 gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc
     9241 atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata
     9301 cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc
     9361 ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc
     9421 catagcctcc gcgaccggtt gtagaacagc gggcagttcg gtttcaggca ggtcttgcaa
     9481 cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctaa actccccaat
     9541 gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc
     9601 tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc
     9661 gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc
     9721 gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc
     9781 tcattgcccc ccggatctgc gaaagctcga gagagataga tttgtagaga gagactggtg
     9841 atttcagcgt gtcctctcca aatgaaatga acttccttat atagaggaag gtcttgcgaa
     9901 ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc
     9961 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
    10021 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga
    10081 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct
    10141 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc
    10201 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
    10261 accatgttat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc
    10321 acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga
    10381 acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact
    10441 gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt
    10501 accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc
    10561 ttggagtaga cgagagtgtc gtgctccacc atgttggcaa gctgctctag ccaatacgca
    10621 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
    10681 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    10741 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    10801 aatttcacac aggaaacagc tatgaccatg attac