Matrices listed below were spiked with certain level of TNFSF4/OX40L and the recovery rates were calculated by comparing the measured value to the expected amount of TNFSF4/OX40L in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-102
95
EDTA plasma(n=5)
89-104
95
heparin plasma(n=5)
88-105
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFSF4/OX40L and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.