Matrices listed below were spiked with certain level of IGFBP-4 and the recovery rates were calculated by comparing the measured value to the expected amount of IGFBP-4 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-104
94
EDTA plasma(n=5)
87-98
92
heparin plasma(n=5)
86-94
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IGFBP-4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.