- $740
- LSBio
- 3.13 - 200 ng/ml
- LS-F21874
- 美国
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Rat
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
3.13 - 200 ng/ml
- 供应商：
康朗生物
- 规格：
96T
Rat Insulin ELISA Kit (Sandwich ELISA)
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Product Description
LS-F21874 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat Insulin in samples of Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of Insulin as low as 3.13 nanograms per millilter.Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
Insulin
Reactivity
Rat
Specificity
This kit recognizes natural and recombinant Rat INS.
Manual
Intended Sample Types
Plasma, Serum
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
3.13 - 200 ng/ml
Sensitivity
3.13 ng/ml
Assay Time
3.5 h
Precision
Intra-Assay: CV<5.23% Inter-Assay: CV<5.5%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Protocol
- Prepare all reagents, samples and standards.
- Add 100 µl standard or sample to each well and incubate for 90 minutes at 37°C.
- Aspirate and add 100 µl prepared Detection Antibody and incubate of 1 hour at 37°C.
- Aspirate and wash 3 times.
- Add 100 µl HRP Conjugate and incubate for 30 minutes at 37°C.
- Aspirate and wash 5 times.
- Add 90 µl Substrate Solution and incubate for 15-20 minutes at 37°C.
- Add 50 µl Stop Solution.
- Read immediately at 450nm.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About Insulin
P01308 NM_000207 NP_000198.1
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Sandwich ELISA Platform Overview
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