重组大肠杆菌GroEL（HSP60）蛋白

产品英文名称：
Recombinant E.Coli Thiol Disulfide Interchange Protein

英文简称:
DsbG

中文名称:
重组大肠杆菌巯基二硫键转换蛋白G

背景资料:
Dsb proteins are in charge for the formation and rearrangement of disulfide bonds during the folding of secreted and membrane proteins in bacteria. DsbG has disulfide bond isomerase and chaperone activity. DsbG interacts with refolding intermediates of chemically denatured citrate synthase and prevents their aMEELPAPVKA IEKQGITIIK TFDAPGGMKG YLGKYQDMGV TIYLTPDGKH AISGYMYNEK GENLSNTLIE KEIYAPAGRE MWQRMEQSHW LLDGKKDAPV IVYVFADPFC PYCKQFWQQA RPWVDSGKVQ LRTLLVGVIK PESPATAAAI LASKDPAKTW QQYEASGGKL KLNVPANVST EQMKVLSDNE KLMDDLGANV TPAIYYMSKE NTLQQAVGLP DQKTLNIIMG NK.regation in vitro. DsbG shares sequnce homology with DsbC. DsbG forms a stable periplasmic dimer and displays an equilibrium constant with glutathione comparable with DsbA and DsbC. DsbG is expressed at roughly 25% level of DsbC.

产品描述:
Recombinant DsbG produced in E.Coli is a single， non-glycosylated polypeptide chain containing 232 amino acids and having a molecular mass of 25.8 kDa. DsbG is purified by conventional chromatography techniques.
来源宿主:
大肠杆菌中重组表达

序列:
MEELPAPVKA IEKQGITIIK TFDAPGGMKG YLGKYQDMGV TIYLTPDGKH AISGYMYNEK GENLSNTLIE KEIYAPAGRE MWQRMEQSHW LLDGKKDAPV IVYVFADPFC PYCKQFWQQA RPWVDSGKVQ LRTLLVGVIK PESPATAAAI LASKDPAKTW QQYEASGGKL KLNVPANVST EQMKVLSDNE KLMDDLGANV TPAIYYMSKE NTLQQAVGLP DQKTLNIIMG NK.

溶解建议:
Sterile filtered colorless solution. The DsbG protein solution contains 20mM Tris-HCl， pH-8， 2mM EDTA and 10% Glycerol.

保存建议：
Store at 4℃ if entire vial will be used within 2-4 weeks. Store， frozen at -20℃ for longer periods of time. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Avoid multiple freeze-thaw cycles.
其他简介：

  ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种最多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。