重组人尿胰蛋白酶抑制剂（UTI）

产品英文名称：
Recombinant Human Ubiquitin Lys 48 to Arg 48 mutation

英文简称:
Ubiquitin K48R

中文名称:
重组人泛素K48R突变

背景资料:
Ubiquitin is a highly conserved 76 amino acid protein expressed in all eukariotes. Ub is found either in frRecombinant human ubiquitin featuring a Lys 48 to Arg48 mutation is useful for the reduction of poly-Ub chain length and conjugation rates. Ubiquitin K48R is expressed in E.coliand purified by proprietary chromatographic techniques. form or conjugated to proteins as monomer or as chain of ubiquitin molecules. The most well characterized consequence of polyubiquitination is substrate degradation， while mono-ubiquitinated proteins are not degraded. Ubiquitin attachment to protein substrate is a complex process involving a ubiquitin activating enzyme （E1）， a ubiquitin conjugating enzyme （E2） and a ubiquitin protein ligase （E3）. The first ubiquitin moiety is transferred to the e-NH2 group of a Lys residue of the protein substrate to generate an isopeptide bond. In successive reactions， a poly ubiquitin chain is synthesized by processive transfer of additional activated moieties to Lys48 of the previously conjugated ubiquitin molecule. Ubiquitin K48R prevents the formation of poly ubiquitin chains via Lys48 linkages with mono ubiquitin molecules， avoiding the degradation of protein substrates.

产品描述:
Recombinant human ubiquitin featuring a Lys 48 to Arg48 mutation is useful for the reduction of poly-Ub chain length and conjugation rates. Ubiquitin K48R is expressed in E.coliand purified by proprietary chromatographic techniques.
来源宿主:
大肠杆菌中重组表达

序列:


溶解建议:
Sterile Filtered clear solution. Diluted in PBS plus 5% glycerol.

保存建议：
Store vial at -20℃ to -80℃. When stored at the recommended temperature， this protein is stable for 12 months. Please prevent freeze-thaw cycles.
其他简介：

  ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种最多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。