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- 供应商：
复祥生物
- 细胞类型：
普通细胞株-科研实验
- 物种来源：
人
- 器官来源：
前列腺
- 运输方式：
常温
- 生长状态：
正常
- 库存：
大量
PC-3 人前列腺癌细胞
F-12培养基+10%胎牛血清。细胞货期8-10个工作日
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CRL-1435 PC-3 人前列腺癌细胞
ATCC® Number: CRL-1435™
Designations: PC-3
Depositors: ME Kaighn
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent (The cells form clusters in soft agar and can be adapted to suspension growth)
Organism: Homo sapiens (human)
Morphology: epithelial
Source: Organ: prostate
Tumor Stage: grade IV
Disease: adenocarcinoma
Derived from metastatic site: bone
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host (Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents)
Tumorigenic: Yes
Antigen Expression: HLA A1, A9
DNA Profile (STR): Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 11
D5S818: 13
D7S820: 8,11
THO1: 6,7
TPOX: 8,9
vWA: 17
Cytogenetic Analysis: The line is near-triploid with a modal number of 62 chromosomes. There are nearly 20 marker chromosomes commonly found in each cell; and normal N2, N3, N4, N5, N12, and N15 are not found. No normal Y chromosomes could be detected by Q-band analysis.
Age: 62 years adult
Gender: male
Ethnicity: Caucasian
Comments: The PC-3 was initiated from a bone metastasis of a grade IV prostatic adenocarcinoma from a 62-year-old male Caucasian. [22363]
The cells exhibit low acid phosphatase and testosterone-5-alpha reductase activities.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
References: 22363: Kaighn ME, et al. Establishment and characterization of a human prostatic carcinoma cell line (PC-3). Invest. Urol. 17: 16-23, 1979. PubMed: 447482
22470: Chen TR. Chromosome identity of human prostate cancer cell lines, PC-3 and PPC-1. Cytogenet. Cell Genet. 62: 183-184, 1993. PubMed: 8428522
26302: Ohnuki Y, et al. Chromosomal analysis of human prostatic adenocarcinoma cell lines. Cancer Res. 40: 524-534, 1980. PubMed: 7471073
32341: Sheng S, et al. Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells. Proc. Natl. Acad. Sci. USA 93: 11669-11674, 1996. PubMed: 8876194
32344: Umekita Y, et al. Human prostate tumor growth in athymic mice: inhibition by androgens and stimulation by finasteride. Proc. Natl. Acad. Sci. USA 93: 11802-11807, 1996. PubMed: 8876218
32460: Carter RE, et al. Prostate-specific membrane antigen is a hydrolase with substrate and pharmacologic characteristics of a neuropeptidase. Proc. Natl. Acad. Sci. USA 93: 749-753, 1996. PubMed: 8570628
32486: Nupponen NN, et al. Genetic alterations in prostate cancer cell lines detected by comparative genomic hybridization. Cancer Genet. Cytogenet. 101: 53-57, 1998. PubMed: 9460501
32488: Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241
32916: Su ZZ, et al. Surface-epitope masking and expression cloning identifies the human prostate carcinoma tumor antigen gene PCTA-1 a member of the galectin gene family. Proc. Natl. Acad. Sci. USA 93: 7252-7257, 1996. PubMed: 8692978
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文献和实验单细胞测序技术（scRNA-Seq）之 NCB 高分文章解析前列腺癌相关研究
单细胞转录组测序，注释细胞类型主要包括上皮细胞、单核-巨噬细胞系、T细胞、内皮细胞、成纤维细胞、肥大细胞等主要群体。然后分别对每个大群进一步分析。 13 例前列腺癌样本的单细胞研究概况 （1）与肿瘤预后相关的上皮细胞类型 通过拷贝数变异分析、肿瘤细胞的marker基因/ 信号通路相关性分析，将上皮细胞鉴定为 luminal types、cell-cycle 、basal/intermediate 类型；我们主要研究了这些细胞类型与临床预后的关系，发现特异性表达细胞因子 CCL2 的basal
发生小的癌灶，发展到老年才出现症状，这一假设尚有待进一步验证。 病变 肉眼观，前列腺癌初期为单个或多数的硬结节，其前列腺可以增大，也可正常大小。早期病灶几乎都发生于包膜下，其中大多数发生于后叶，其次是两侧及前叶的包膜下，而发生于中叶者极为少见。晚期肿瘤可扩展到全部前列腺，使前列腺明显增大而质地变硬。切面灰白色夹杂以多少不等的纤维性条纹或间隔，也可呈均质性夹以不规则的黄色区域。 镜下，97％的前列腺癌均为腺癌，少数为移行细胞癌和鳞状细胞癌。依其分化程度可分为高分化、中分化和低分
丁香园网友kj7156084的培养方法： 本室的PC－3来自上海细胞生物所，种是ATCC的。 以10％FBS＋1640或F12培养，0.25%胰酶消化。塑料瓶或玻璃瓶都可以，当然，前者效果较好。复苏时最好用塑料瓶。 5－7天传一次（1：2），2－3天换一次液。尽量高密度传，因为PC－3细胞喜欢扎堆长，如果过了两三天细胞长得不均匀，可以消化离心一下。 传代后一到两天不要动它，要有耐心。 人前列腺癌细胞PC－3（高密
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