Panc 04.03 人胰腺癌细胞

Panc 04.03 人胰腺癌细胞

收藏
  • 询价
  • ATCC
  • Panc 04.03
  • 2025年12月07日
    avatar
    上海复祥生物科技有限公司
    13 年金牌会员

  • 企业认证

    点击 QQ 联系

    • 相关产品推荐更多 >

    万千商家帮你免费找货

    0 人在求购买到急需产品
    • 详细信息
    • 技术资料
    • 库存：

      大量

    • 是否是肿瘤细胞：

      0

    ATCC® Number: CRL-2555™ Price: $429.00
    Designations: Panc 04.03
    Depositors: EM Jaffee
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Homo sapiens (human)
    Morphology: epithelial
    Source: Organ: pancreas
    Disease: adenocarcinoma
    Cellular Products: cytokeratins 7 and 18 [50655]
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    CRL-2555 Panc 04.03 人胰腺癌细胞
    Isolation: Isolation date: April 3, 1995
    Tumorigenic: Yes
    Oncogene: K-ras +
    Antigen Expression: MHC class I +; MHC class II - [50655]
    Blood type O; Rh+
    DNA Profile (STR): Amelogenin: X
    CSF1PO: 12
    D13S317: 11,12
    D16S539: 11,12
    D5S818: 11
    D7S820: 8,12
    THO1: 9.3
    TPOX: 10,11
    vWA: 14,16
    Age: 70 years adult
    Gender: male
    Ethnicity: White
    Comments: Panc 04.03 is a pancreatic adenocarcinoma epithelial cell line derived, in 1995, from a primary tumor removed from the head-of-the-pancreas of a male with pancreatic adenocarcinoma.
    The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655]
    The cells have a reported plating efficiency of 80%. [50655]
    Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 20 Units/ml human recombinant insulin, 85%; fetal bovine serum, 15%
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C CRL-2555 Panc 04.03 人胰腺癌细胞
    Subculturing: Protocol:
    1. Remove and discard culture medium.
      • Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
        • Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
          Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
          • Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
            • Add appropriate aliquots of the cell suspension to new culture vessels.
              • Incubate cultures at 37°C.

    Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
    Medium Renewal: Add media once per week. Fluid change one to two times per week.
    Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Doubling Time: 50.5 hrs
    Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
    recommended serum:ATCC 30-2020
    References: 50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602CRL-2555 Panc 04.03 人胰腺癌细胞

    风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。

    图标技术资料

    需要更多技术资料 索取更多技术资料

    资料下载：

    logo-4.jpg 附 (下载 0 次)

    同类产品报价

    产品名称
    产品价格
    公司名称
    报价日期
    Panc04.03细胞
    ¥900
    上海泽叶生物科技有限公司
    2025年12月16日询价
    Panc04.03细胞
    ￥900
    上海淳麦生物科技有限公司
    2025年07月09日询价
    Panc04.03细胞
    询价
    上海沪震实业有限公司
    2025年12月17日询价
    Panc 04.03细胞、Panc 04.03 胰腺腺癌细胞
    ￥1200
    上海酶研生物科技有限公司
    2025年07月11日询价
    Panc04.03人胰腺癌细胞
    ￥880
    武汉华尔纳生物科技有限公司
    2025年07月13日询价
    Panc 04.03 人胰腺癌细胞
    询价