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Panc 04.03 人胰腺癌细胞

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  • Panc 04.03
  • 2025年12月07日
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    上海复祥生物科技有限公司
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    ATCC® Number: CRL-2555™ Price: $429.00
    Designations: Panc 04.03
    Depositors: EM Jaffee
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Homo sapiens (human)
    Morphology: epithelial
    Source: Organ: pancreas
    Disease: adenocarcinoma
    Cellular Products: cytokeratins 7 and 18 [50655]
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    CRL-2555 Panc 04.03 人胰腺癌细胞
    Isolation: Isolation date: April 3, 1995
    Tumorigenic: Yes
    Oncogene: K-ras +
    Antigen Expression: MHC class I +; MHC class II - [50655]
    Blood type O; Rh+
    DNA Profile (STR): Amelogenin: X
    CSF1PO: 12
    D13S317: 11,12
    D16S539: 11,12
    D5S818: 11
    D7S820: 8,12
    THO1: 9.3
    TPOX: 10,11
    vWA: 14,16
    Age: 70 years adult
    Gender: male
    Ethnicity: White
    Comments: Panc 04.03 is a pancreatic adenocarcinoma epithelial cell line derived, in 1995, from a primary tumor removed from the head-of-the-pancreas of a male with pancreatic adenocarcinoma.
    The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655]
    The cells have a reported plating efficiency of 80%. [50655]
    Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 20 Units/ml human recombinant insulin, 85%; fetal bovine serum, 15%
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C CRL-2555 Panc 04.03 人胰腺癌细胞
    Subculturing: Protocol:
    1. Remove and discard culture medium.
      • Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
        • Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
          Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
          • Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
            • Add appropriate aliquots of the cell suspension to new culture vessels.
              • Incubate cultures at 37°C.

    Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
    Medium Renewal: Add media once per week. Fluid change one to two times per week.
    Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Doubling Time: 50.5 hrs
    Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
    recommended serum:ATCC 30-2020
    References: 50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602CRL-2555 Panc 04.03 人胰腺癌细胞

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      （图03-2）又叫舌背，舌背上有一向前开放的“V”型沟叫界沟，将舌分为前2/3的舌体和后1/3的舌根。舌体的前端叫舌尖，舌根对向口咽部。舌下面较舌背短，粘膜光滑而松软，与口底粘膜相续，在正中线上的粘膜皱襞即舌系带。 舌粘膜：舌体粘膜上有密集的小突起叫舌乳头，根据其形态可将舌乳头分为4类：①丝状乳头：细而长，呈白色丝绒状，遍布舌体表面，由于其浅层上皮细胞不断角化脱落，并和食物残渣共同附着在舌粘膜的表面形成舌苔，健康人舌苔很淡薄。②菌状乳头：散在于丝状乳头之间，顶端稍膨大而钝圆，肉眼看呈红色

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