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- 详细信息
- 文献和实验
- 技术资料
- 形态:
液体
- 保存条件:
4度
- 克隆性:
Polyclonal Antibody
- 标记物:
Purified
- 适应物种:
Human
- 宿主:
Goat
- 应用范围:
E, WB
- 浓度:
请参考说明书
- 靶点:
IgE
- 抗体英文名:
GOAT ANTI DOG IgE
- 抗体名:
GOAT ANTI DOG IgE
- 规格:
0.5 mg
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文献和实验Purifying and Measuring Immunoglobulin E (IgE) and Anti-IgE
to anaphylaxis. Here, we describe the history behind the IgE discovery and the protocol for purifying IgE and anti-IgE in the mouse. This is followed by our ELISA protocol for mouse IgE detection.
and wash the blot 3 times, 5-7 minutes each, with 0.5% BSA in TBST.11. Incubate with Goat anti-Rabbit/alkaline phosphatase (from Promega; 1:7500 inTBST with 5% BSA) for 1 hour at room temp with shaking. I have also used successfully Amersham ECL detection
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
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