- ￥2980
- Sino Biological
- 50095-HNAE
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 规格：
50 µg
蛋白名称：IL36 alpha/IL-1F6蛋白, IL36 alpha/IL-1F6 protein
蛋白构建：A DNA sequence encoding the mouse IL1F6 (Q9JLA2-1) (Met 1-His 160) was expressed and purified.
表达宿主：E. coli
蛋白纯度：> 97 % as determined by SDS-PAGE
蛋白活性：0
蛋白内毒素：Please contact us for more information.
预测N端：Met 1
蛋白分子量：The recombinant mouse IL1F6 consisting of 160 amino acids and migrates with an apparent molecular mass of 18 kDa as predicted.
蛋白NP号：Q9JLA2-1
蛋白氨基酸序列：Met1-His160
蛋白标签：Native
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验LABELING ALPHA-ACTININ WITH IATR
with good stirring.The volume (ml)of borate buffer = mg IATR x 9 / (mg/ml)alpha-actinin. 4.Clarify IATR solution at 25,000 rpm,4℃,for 10 min in a 42.2Ti rotor. 5.Mix equal volumes of clarified dye and alpha-actinin in a test tube; remove
ALPHA FACTOR ARREST AND RELEASE
of approximately 3 x 10�5 M, while bar1 strains require much lower pheromone concentrations (10-8 M). To release cells from alpha factor arrest, spin down cells and wash twice (using a large volume, dependent on the size of the cell pellet anticipated
In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
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