- ￥32380
- Sino Biological
- 北京
- 10440-H08H-UE
- 2026年01月26日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human Alkaline Phosphatase / ALPL Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1 mg
蛋白名称：Human Alkaline Phosphatase / ALPL Protein (His Tag), Endotoxin-Free
蛋白构建：A DNA sequence encoding the human tissue-nonspecific alkaline phosphatase (NP_000469.3) (Met 1-Ser 502) was expressed with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：≥ 95 % as determined by SDS-PAGE.
蛋白内毒素：< 0.5 EU per mg protein.
预测N端：Leu 18
蛋白分子量：The recombinant human ALPL consists of 496 amino acids and predicts a molecular mass of 55 kDa. As a result of glycosylation, it migrates as an approximately 62-78 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_000469.3
蛋白氨基酸序列：Met 1-Ser 502
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文献和实验Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
Materials Anti-digoxigenin-AP conjugate (Roche #11093274910, 150U/200 uL, $168) Levamisole (Sigma #T1512, 2 g, $10) (blocks endogenous phosphatase activity) BCIP (Roche #11383221001, 3 mL, $49) NBT (Roche #11383213001, 3 mL, $48)
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of X-gal can be easier to detect prior to carrying out the X-Phos reaction as background alkaline phosphatase staining can obscure it, particularly in whole mounts. Rinse the tissue very well with PBS prior to PLAP staining as residual ß-gal activity
Purification of acidic phosphatase from mustard seedlings
at which such phosphatases have optimal activity, we distinguish between acidic phosphatases (also called acid phosphatases) and alkaline phosphatases. The latter enzymes require divalent metal ions as cofactors and are common in animal tissues and bacteria. Acidic
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