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100 µg
蛋白名称:HIST3H2A蛋白, HIST3H2A protein
蛋白构建:A DNA sequence encoding the human HIST3H2A (NP_254280.1) (Met 1-Lys 130) was expressed and purified.
表达宿主:E. coli
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:Please contact us for more information.
预测N端:Met 1
蛋白分子量:The recombinant human HIST3H2A consists of 130 amino acids and has a calculated molecular mass of 14.2 kDa as estimated in SDS-PAGE under reducing conditions.
蛋白NP号:NP_254280.1
蛋白氨基酸序列:Met1-Lys130
蛋白标签:Native
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验the 3 hour incubation Dynabeads™ M-280 Streptaviden magnetic beads (7x107 beads, ~100µl/sample) are added to the respective samples (note 1);5.The resultant siRNA/Flag complexes are then eluted with magnetic bead binding, the solution can be discarded
, for example, this protocol yields ~2 x 109 nuclei/g tissue. Nuclei Preparation from Cultured Cells (2 h) 9. Culture 1 x 107 to 1 x 108 cells. Ensure that the cells are not grown beyond semiconfluency. 10. Rinse
an excellent method to separate not only the different modified forms of histones, but also the primary sequence variant subtypes of selected histone species; it is widely used to separate histones with varying levels of acetylation. This unit describes the use
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