- ￥3870
- Sino Biological
- 北京
- 13096-H09E
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Human HNMT / Histamine N-methyltransferase Protein (GST Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
50 µg
蛋白名称：Human HNMT / Histamine N-methyltransferase Protein (GST Tag)
蛋白构建：A DNA sequence encoding the human HNMT (AAH20677.1) (Met 1-Ala 292) was fused with the GST tag at the N-terminus.
表达宿主：E. coli
蛋白纯度：> 85 % as determined by SDS-PAGE
蛋白活性：0
蛋白内毒素：Please contact us for more information.
预测N端：Met
蛋白分子量：The recombinant human HNMT/GST chimera consists of 524 amino acids and has a predicted molecular mass of 60.5 kDa. It migrates as an approxiamtely 50 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：AAH20677.1
蛋白氨基酸序列：Met1-Ala292
蛋白标签：N-GST
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验GST fusion protein purification GST融合蛋白纯化
1)grow 20ml cells O/N37℃ dilute 50X into prewarmed LB,grow to 0.6 OD or about 1hr.Induce w/ 2mM IPTG (238mg/0.5l),grow 3hr,spin 6k GS3 10',freeze at 70℃2)extract cells (from 500ml)in 25 mls.Heintz Buffer plus triton (HBT)by gentle pipette
GST fusion protein purification--GST融合蛋白纯化
GST fusion protein purification GST融合蛋白纯化John Mundy,Institute of Molecular Biology,Copenhagen,Denmark1)grow 20ml cells O/N37℃ dilute 50X into prewarmed LB,grow to 0.6 OD or about1hr.Induce w/ 2mM IPTG (238mg/0.5l),grow 3hr,spin 6k GS3 10',freeze
GST融合蛋白的准备 Preparation of Glutathione
相关专题 Margret B. Einarson and Elena N. Pugacheva Foxx Chase Cancer Center, Philadelphia, PA 19111 Jason R. Orlinck Brigham & Women"s Hospital, Boston, MA 02115 Excerpted from Protein: Protein Interactions
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