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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Human HNMT / Histamine N-methyltransferase Protein (GST Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
50 µg
蛋白名称:Human HNMT / Histamine N-methyltransferase Protein (GST Tag)
蛋白构建:A DNA sequence encoding the human HNMT (AAH20677.1) (Met 1-Ala 292) was fused with the GST tag at the N-terminus.
表达宿主:E. coli
蛋白纯度:> 85 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:Please contact us for more information.
预测N端:Met
蛋白分子量:The recombinant human HNMT/GST chimera consists of 524 amino acids and has a predicted molecular mass of 60.5 kDa. It migrates as an approxiamtely 50 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:AAH20677.1
蛋白氨基酸序列:Met1-Ala292
蛋白标签:N-GST
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Histamine N-Methyltransferase: Enzymology and Functional Aspects
Histamine N -methyltransferase (HNMT; EC 2.1.1.8) was discovered a little over 25 yr ago. Since that time, HNMT has been the subject of numerous investigations, and although these studies have provided a fundamental understanding of this enzyme
GST fusion protein purification--GST融合蛋白纯化
GST fusion protein purification GST融合蛋白纯化John Mundy,Institute of Molecular Biology,Copenhagen,Denmark1)grow 20ml cells O/N37℃ dilute 50X into prewarmed LB,grow to 0.6 OD or about1hr.Induce w/ 2mM IPTG (238mg/0.5l),grow 3hr,spin 6k GS3 10',freeze
亲和力在微摩尔范围。一些融合蛋白在0.2% Triton X-100或0.25% Tween 20存在下不能有效结合,而其他融合蛋白则不受影响。 缓冲条件为pH7.0到8.5,盐浓度可高达1M,但不能使用变性剂。如果要去除MBP融合部分,可用位点特异性蛋白酶切除。检测:可用MBP抗体或表达的目的蛋白特异性抗体检测。GST:GST(谷胱甘肽巯基转移酶) 标签蛋白本身是一个在解毒过程中起到重要作用的转移酶,它的天然大小为26KD。将它应用在原核表达的原因大致有两个,一个是因为它是一个高度可溶的蛋白
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