- ￥1080 - 23190
- Sino Biological
- 北京
- 80012-RNAE
- 2025年08月12日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Rat CNTF / Ciliary Neurotrophic Factor Protein
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20 µg/100 µg/1 mg
| 规格： | 20 µg | 产品价格： | ￥1080.0 |
|---|---|---|---|
| 规格： | 100 µg | 产品价格： | ￥3980.0 |
| 规格： | 1 mg | 产品价格： | ￥23190.0 |
蛋白名称：Rat CNTF / Ciliary Neurotrophic Factor Protein
蛋白构建：A DNA sequence encoding the rat CNTF (P20294) (Met 1-Met 200) was expressed and purified.
表达宿主：E. coli
蛋白纯度：> 92 % as determined by SDS-PAGE
蛋白活性：1. Measured by its binding ability in a functional ELISA. Immobilized rat CNTF (Cat: 80012-RNAE) at 10 μg/ml (100 μl/well) can bind biotinylated rat CNTFR-His (aa 1-346) (Cat: 80019-R08B) with a linear range of 1.56-50.0 ng/ml. 2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 30-120 ng/ml.
蛋白内毒素：Please contact us for more information.
预测N端：Met 1
蛋白分子量：The recombinant rat CNTF consisting of 200 amino acids and has a calculated molecular mass of 22.9 kDa. It migrates as an approximately 24 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：P20294
蛋白氨基酸序列：Met1-Met200
蛋白标签：Native
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Expression of Neurotrophic Factors such as GDNF in the E. coli System
neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5, glial-cell-line-derived neurotrophic factor (GDNF), ciliary neurotrophic factor (CNTF), insulin-like growth factor-1 (IGF-I), are currently in clinical trials and have potential
Identification of Neurotrophic Factors by Molecular Biological Techniques
factor (BDNF), and ciliary neurotrophic factor (CNTF) were discovered by their specific neurotrophic properties. They were purified from tissue extracts using in vitro bioassays based on the survival of specific nerve cells and subsequently
X-100或脱氧胆酸钠）和低浓度变性剂（2mol/L尿素或盐酸胍等）洗涤除去脂类和膜蛋白，这一步很重要，否则会导致包涵体溶解和复性的过程中重组蛋白质的降解[6、7、8]。 包涵体的溶解必须用很强的变性剂，如8mol/L尿素、6~8mol/L盐酸胍，通过离子间的相互作用破坏包涵体蛋白间的氢键而增溶蛋白。其中尿素的增溶效果稍差，异氰盐酸胍最强；去污剂，如SDS[7]，可以破坏蛋白内的疏水键，可以增溶几乎所有的蛋白，但由于无法彻底去除而不允许用在制药行业中；酸，如70%甲酸[9]，可以破坏蛋白
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