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- Sino Biological
- 10902-RP01
- 2025年08月12日
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100 µL
抗体种属：Human
抗体靶点：CCNE1
抗体应用：ELISA
抗原货号：10902-H07B
抗原描述：Recombinant Human CCNE1 protein (Catalog#10902-H07B)
抗体宿主：Rabbit
抗体Ig类型：Rabbit IgG
抗体纯化方法：Protein A
抗体制备：Produced in rabbits immunized with purified, recombinant Human CCNE1 (rh CCNE1 , Catalog#10902-H07B; NP_001229.1; Met 1-Ala 410). Total IgG was purified by Protein A affinity chromatography .
抗体特异性：Human CCNE1 / Cyclin-E1
抗体保存：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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文献和实验Assays for Cyclin-Dependent Kinase Inhibitors
of the Retinoblastoma gene product (Rb) by CDK4 and/or CDK6 (G1/S checkpoint), leading to the release of bound E2F from Rb and allowing the transcription of genes by “free E2F” necessary for S phase progression. During late G1 phase, CDK2/cyclin E complex phosphorylates
E2F Transcription Factors and pRb Pocket Proteins in Cell Cycle Progression
The E2F-family of transcripion factors exerts fascinating and contrasting functions in tran-scriptional repression and activation of genes regulating proliferation, apoptosis, and differentiation. E2F is principally regulated by its temporal
DNA(经PI-SceⅠ和I-CeuⅠ预消化为线形DNA)连接。为了提高筛选效率，用SwaⅠ酶切连接产物以除去自身环化或者其他可能存在的非线性的腺病毒DNA（SwaⅠ酶切位点在环状Adeno-X病毒DNA的PI-SceⅠ和I-CeuⅠ位点之间）。用此DNA转化大肠杆菌DH5α并筛选重组子。最后，用重组腺病毒DNA转染HEK 293病毒包装细胞系。由于从Adeno-X病毒DNA中删除了E1基因，使其无法感染细胞，这一步需要用HEK 293包装细胞提供反式的E1蛋白，即可得到含有目的基因、有感染能力的重组腺病
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