Recombinant Rat E-Selectin / CD62e / SELE Protein (His Tag)
库存:
99
供应商:
北京义翘神州科技股份有限公司
规格:
100 µg
重组大鼠 E-Selectin / CD62e / SELE 蛋白 (His标签)(产品说明)
蛋白名称:Rat E-Selectin / CD62e / SELE Protein (His Tag)
蛋白构建:A DNA sequence encoding the rat SELE (P98105-1) extracellular domain (Met 1-Pro 494) was expressed, fused with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 97 % as determined by SDS-PAGE
蛋白活性:Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to rat E Selectin/Fc Chimera coated plates (2 μg/mL, 100 μL/well), approximately 30%-60% will adhere after 1 hour at 37℃. Optimal dilutions should be determined by each laboratory for each application.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Trp 22
蛋白分子量:The recombinant rat SELE comprises 484 amino acids and predicts a molecular mass of 53 kDa. The apparent molecular mass of the rat SELE is approximately 70-80 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号:P98105-1
蛋白氨基酸序列:Met1-Pro494
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
1, Kwon, S; et al. An inflammatory vascular endothelium-mimicking microfluidic device to enable leukocyte rolling and adhesion for rapid infection diagnosis. Biosensors and Bioelectronics