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- Sino Biological
- 北京
- 11676-VNAHC1
- 2026年01月26日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Influenza A H5N1 Neuraminidase / NA (H255Y) (Active)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
60.00 U/300.00 U
| 规格： | 60.00 U | 产品价格： | ￥800.0 |
|---|---|---|---|
| 规格： | 300.00 U | 产品价格： | ￥2400.0 |
蛋白名称：Neuraminidase/NA蛋白, Neuraminidase/NA protein
蛋白构建：A DNA sequence encoding the Influenza A virus (A/Anhui/1/2005 (H5N1)) neuraminidase (ABU94738.1) (Met 1-Lys 449) was expressed, the cell lysates are collected, and bio-activity was tested. There is an amino acid change from Histidine to Tyrosine (H255Y) in NA / Neuraminidase.
表达宿主：HEK293 Cells
蛋白纯度：0
蛋白活性：Measured by its ability to cleave a fluorogenic substrate, 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid. The specific activity is > 60 U. The specific activity is > 300 U. One unit is defined as the amount of enzyme required to cleave 1 nmole of 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid per minute at pH 7.5 at 37℃.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：0
蛋白分子量：The recombinant influenza A H5N1 Neuraminidase comprises 449 amino acids and has a predicted molecular mass of 49.2 kDa.
蛋白NP号：ABU94738.1
蛋白氨基酸序列：Met1-Lys449
蛋白标签：Native
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验The Mouse as Model System to Study Host‐Pathogen Interactions in Influenza A Infections
. Gambotto, A., Barratt‐Boyes, S.M., de Jong, M.D., Neumann, G., and Kawaoka, Y. 2008. Human infection with highly pathogenic H5N1 influenza virus. Lancet 371:1464‐1475.
tube: sterile ddH20 q.s (where "q.s." means quantity sufficient)10X assay buffer one-tenth volumeDNA x ulrestriction enzyme* y ul (1-10 units per ug DNA)Total volume z ul *If desired
in the order listed to a microcentrifuge tube: sterile ddH2O q.s (where "q.s." means quantity sufficient) 10X assay buffer one-tenth volume DNA x μl restriction enzyme* y μl (1-10 units per ug DNA) Total volume z μl
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