Human IL2RA / CD25 ELISA Kit (

Human IL2RA / CD25 ELISA Kit (Sandwich ELISA)

Product Description

LS-F36095 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human IL2RA / CD25 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of IL2RA / CD25 as low as 56.25 picograms per milliliter.

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Specifications

Type
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
IL2RA / CD25
Reactivity
 
Human
Manual
 

Intended Sample Types
 
Plasma, Serum, Tissue Homogenates
Format
 
96-Well Strip Plate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
93.75 - 6000 pg/ml
Sensitivity
 
56.25 pg/ml
Precision
 
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
 
Store at 4°C. Stable for 6 months.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• User Manual
• 96-well Microplate
• Detection Reagents
• Wash Buffer
• Substrate
• Stop Solution
• Adhesive Plate Sealers

Background
 
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CD25antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CD25 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP- Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CD25 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CD25 can be calculated.
Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio 100% Guarantee.
About IL2RA / CD25
 
Uniprot: P01589 NCBI: NM_000417 NP_000408.1