Rat IL6 / Interleukin 6 ELISA

Rat IL6 / Interleukin 6 ELISA Kit (Sandwich ELISA)

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LS-F10479-1
1 plate
$675 USD
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Product Description

LS-F10479 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat IL6 / Interleukin 6 in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of IL6 / Interleukin 6 as low as 5 picograms per milliliter.

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Specifications

Type
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
IL6 / Interleukin 6
Reactivity
 
Rat
Specificity
 
This kit is for the detection of natural and recombinant rat IL-6. No significant cross-reactivity or interference between IL-6 and analogs was observed.
Manual
 

Intended Sample Types
 
Cell Culture Supernatants, Plasma, Serum
Format
 
96-Well Microplate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
62.5 - 4000 pg/ml
Sensitivity
 
5 pg/ml
Precision
 
Intra-Assay: CV%<9.1% Inter-Assay: CV%<9.5%
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• User Manual
• 96-well Microplate
• Detection Reagents
• Wash Buffer
• Substrate
• Stop Solution
• Adhesive Plate Sealers

Background
 
Interleukin-6(IL-6) is a protein that in humans is encoded by the IL6 gene. Interleukin-6(IL-6) is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. IL-6 is a pleiotropic cytokine that plays a critical role in bone resorption. IL-6 has been suggested to stimulate the HPA axis during immune activation independent of the input of hypothalamic corticotropin-releasing hormone(CRH). Interleukin-6(IL-6) is an immunoregulatory cytokine that activates a cell-surface signaling assembly composed of IL-6, the IL-6 alpha-receptor(IL-6Ralpha), and the shared signaling receptor gp130. The standard product used in this kit is recombinant rat IL-6, consisting of 188 amino acids with the molecular mass of 21.8kDa.
Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio 100% Guarantee.
About IL6 / Interleukin 6
 
Uniprot: P05231 NCBI: NM_000600 NP_000591.1

 

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