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文献和实验Denaturing Urea-Polyacrylamide Gel Electrophoresis (PAGE) Based Microsatellite Analysis
. Use a needle attached to the syringe filled with 1x-TBE buffer to flush out all the wells. Denature PCR products (5 μl) along with 10-bp ladder mixed in 2X loading dye (20 mM EDTA, 0.05% Xylene cyanole, prepared in 95%formamide
Radiolabeled sequencing gel preparation, loading, and electrophoresis
to the buffer chambers. The wells are cleaned by circulating buffer into the wells with a syringe and, immediately prior to the loading of each sample, the urea in each well is suctioned out with a mouth pipette. Each base-specific sequencing reaction
ChIP Protocol-Mechanical Breakage & FA Lysis Buffer
. 2. Sonicate leftover FA lysis buffer w/ supplements for a moment. 3. Put open sample tube in round, cold rack (from freezer) and sonicate for 10 seconds. Avoid aerating the sample. 4. Let sample sit on ice for 5 minutes. Dave's Note: ice time
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