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- 技术资料
- 供应商：
简石生物
- 保存条件：
常温
新品试用
1、本产品可长期试用，申请 DNA/RNA 保护剂 同步获得总 RNA 提取试剂盒(配合TRIZOL使用)
2、递交试用报告通过审核的，可获得精美 U 盘一份。
DNA/RNA保护剂（DNA/RNA Shield）
| 货号 | 规格 |
| TR110-50 | 50ml |
| TR110-250 | 250ml |
| TR120-25 | 25ml |
| TR120-125 | 125ml |
产品简介：
- 保存各种样品中基因的完整性（细胞，组织，唾液，血液，液体样品，粪便，等等）；
- DNA和RNA可以直接分离得到而不需要去离心沉淀或去除试剂；
- 完全灭活各种微生物（病毒，细菌，酵母菌）；
- 常温保存样品，提供了最佳的微生物特征稳定性。
特性：
- DNA/RNA Shield可以保证样品；
- 在常温运输时核酸的稳定；
- 无需冷链运输或特殊的设备。
- DNA/RNA Shield可有效裂解细胞；
- 灭活各种核酸酶和传染性的微生物（病毒，细菌，酵母）；
- 兼容各种的样品保存装置。
DNA/RNA Shield 与样品比例说明:
在一个合适的管子或者样品采集设备中按照比例添加核酸保护剂并且匀浆。
| TR110 | 固体保护剂 |
| DNA/RNA Shield | 针对固体组织（细胞，组织，口腔拭子，粪便等样） |
| 样品体积不超过核酸保护剂总体积的10% | |
| TR120 | 液体保护剂 |
| DNA/RNA Shield | 针对液体样品（血液，唾液，脑脊液，羊水等样） |
| 添加同等体积（1：1）的核酸保护剂到样品中 | |
添加保护剂后的样品储存时效性说明：
| DNA | 常温可稳定30天 -20℃或者更低的温度可以稳定1年以上 |
| RNA | 37℃下稳定3天 4-25℃稳定30天 -20℃或者更低的温度可以稳定1年以上 |
DNA / RNA Shield™提供了最佳的微生物特征稳定性
研究人员建议保留DNA / RNA Shield™中的所有样本，以限制大规模人类微生物组研究中的收集和储存偏差。
保护剂对应的其他产品：
保护剂处理后的 核酸提取：
添加了DNA/RNA保护剂的样品，下游可使用市面上大部分的核酸提取纯化试剂盒
针对不同样品的核酸提取选择，我们更推荐使用天漠生物的核酸提取试剂盒进行下一步的提取纯化
(详情请咨询我们的工作人员)
诚招各地经销商及合作伙伴
使用此产品的优质用户；
证书：
https://www.zymoresearch.com/pages/25-anniversary?pages=space
该产品被引用文献：
1、DNA/RNA Shield was used to store water collected onto filters and total RNA was isolated. A detailed metatranscriptomic analysis of the microbial communities points towards differing metabolic cycles occurring in anoxic and saline habitats in upper and deep sea regions respectively.
Pachiadaki MG, et. al. (2014). Unveili
https://www.ncbi.nlm.nih.gov/pubmed/24950109
2、FACS sorted cells derived from disassociated 3D tissue matrix were collected in DNA/RNA Shield for downstream RNA-seq. Transcriptome analyses revealed that modifying crosslinking of hydrogels can optimize expression patterns to correlate with specific brain regions in an engineering 3D neural tissue.
Tekin, H et al. Effects of 3D culturing conditions on the transcriptomic profile of stem-cell-derived neurons. Nature Biomedical Engineering. 2018.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6157920/
3、DNA/RNA Shield was used to collect biomass samples from bench-scale microbial fuel cells. After bead-beating homogenization, the supernatant was used for automated DNA and RNA extraction on a Promega Maxwell Magnetic Particle Processor with the Maxwell 16 LEV Blood DNA and 16 LEV simplyRNA Blood kits.
Chen, S et al. Methane-driven microbial fuel cells recover energy and mitigate dissolved methane emissions from anaerobic effluents. Environmental Science: Water Research & Technology. 2018.
https://pubs.rsc.org/en/content/articlelanding/2018/ew/c7ew00293a#!divAbstract
使用此产品的优质用户；
证书：
https://www.zymoresearch.com/pages/25-anniversary?pages=space
该产品被引用文献：
1、DNA/RNA Shield was used to store water collected onto filters and total RNA was isolated. A detailed metatranscriptomic analysis of the microbial communities points towards differing metabolic cycles occurring in anoxic and saline habitats in upper and deep sea regions respectively.
Pachiadaki MG, et. al. (2014). Unveili
https://www.ncbi.nlm.nih.gov/pubmed/24950109
2、FACS sorted cells derived from disassociated 3D tissue matrix were collected in DNA/RNA Shield for downstream RNA-seq. Transcriptome analyses revealed that modifying crosslinking of hydrogels can optimize expression patterns to correlate with specific brain regions in an engineering 3D neural tissue.
Tekin, H et al. Effects of 3D culturing conditions on the transcriptomic profile of stem-cell-derived neurons. Nature Biomedical Engineering. 2018.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6157920/
3、DNA/RNA Shield was used to collect biomass samples from bench-scale microbial fuel cells. After bead-beating homogenization, the supernatant was used for automated DNA and RNA extraction on a Promega Maxwell Magnetic Particle Processor with the Maxwell 16 LEV Blood DNA and 16 LEV simplyRNA Blood kits.
Chen, S et al. Methane-driven microbial fuel cells recover energy and mitigate dissolved methane emissions from anaerobic effluents. Environmental Science: Water Research & Technology. 2018.
https://pubs.rsc.org/en/content/articlelanding/2018/ew/c7ew00293a#!divAbstract
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【免费试用】DNA/RNA Shield 样品保护剂
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