Cyclin E1 Recombinant Rabbit mAb(bsm-52048R)-50ul/100ul产品图
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Cyclin E1 Recombinant Rabbit m

Ab(bsm-52048R)-50ul/100ul
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  • ￥1400 - 2500
  • Bioss已认证
  • bsm-52048R
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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      50ul/100ul

    规格：50ul产品价格：￥1400.0
    规格：100ul产品价格：￥2500.0
    产品编号bsm-52048R
    英文名称Cyclin E1 Recombinant Rabbit mAb
    中文名称周期素E重组兔单抗
    英文别名CCNE 1; CCNE; CCNE1; Cyclin Es; Cyclin Et; CyclinE; G1/S specific cyclin E; G1/S-specific cyclin-E1; CCNE1_HUMAN; pCCNE1.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human
    抗体来源Rabbit
    免疫原A synthesized peptide derived from human Cyclin E1
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Recombinant
    理论分子量45 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cancer > Cell cycle > Cyclins > Cyclin E family

    Cell Biology > Cell Cycle > Cyclins

    Cell Biology > Cell Cycle > Cyclins > Cyclin E Family

    Epigenetics and Nuclear Signaling > Cell cycle > Cyclins > Cyclin E Family

    亚基 Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation.
    亚细胞定位Nucleus.
    组织特异性Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
    翻译后修饰Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
    相似性Belongs to the cyclin family. Cyclin E subfamily.
    功能Essential for the control of the cell cycle at the G1/S (start) transition.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {ICC/IF}{1:50-200}
    {IF}{1:100-500}

     

    产品细节图片1
    25 ug total protein per lane of various lysates (see on figure) probed with Cyclin E1 monoclonal antibody, unconjugated (bsm-52048R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cyclin E1 Monoclonal Antibody, Unconjugated (bsm-52048R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cyclin E1 Monoclonal Antibody, Unconjugated (bsm-52048R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片4
    P‌‌araformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cyclin E1 Monoclonal Antibody, Unconjugated (bsm-52048R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片5
    P‌‌araformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cyclin E1 Monoclonal Antibody, Unconjugated (bsm-52048R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片6
    P‌‌araformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cyclin E1 Monoclonal Antibody, Unconjugated (bsm-52048R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

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    图标文献和实验
    该产品被引用文献

    [IF={{ 3.738 }}] {Qiao Zhang. et al. G6PD upregulates Cyclin E1 and MMP9 to promote clear cell renal cell carcinoma progression. Int J Med Sci. 2022; 19(1): 47–64} {IHC} {Human}

    [IF={{ 2.6 }}] {Zhao Xiaodong. et al. The Regulation of ZAR1 on Apoptosis and Mitophagy in Ovarian Granular Cells and Primary Ovarian Insufficiency (POI) Mice. REPROD SCI. 2025 Apr;:1-13} {WB} {Human,Mouse}

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    Cyclin E1 Recombinant Rabbit mAb(bsm-52048R)-50ul/100ul
    ￥1400 - 2500