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技术
electrophoresis: suitable
适用性
suitable for electrophoresis
异质活性
Protease, none detected
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文献和实验Separation and Detection of Phosphorylated and Nonphosphorylated BvgA, a Bordetella pertussis Response Regulator, in vivo and in vitro
Chen Q, et al.
Bio-protocol, 3(22) (2013)
The ribonuclease protection assay (RPA)
. Siliconize the short plate and clean again. Assemble the gel mold (0.4 mm spacers). 22. Combine the following to give a final concentration of 5% acrylamide: 74.5 ml acrylamide solution (final 19:1 acrylamide/bis): 8.85 mls of 40
ELECTROPHORESIS OF DNA IN POLYACRYLAMIDE GELS
all of it has been transferred from the stock bottle. 30% Acrylamide Solution (0.8% bis) 60 g acrylamide 1.6 g bis Heat to dissolve in approximately 100 ml of water. QS to 200 ml with water and filter with Whatman #1 filter paper into a foil
Preparation of Polyacrylamide Gels
1. Prepare 20X TBE as: 216 g Tris Base 110 g Boric Acid 80 mL 500 mM EDTA, pH 8.0 700 mL ddH2O Mix. Bring volume to 1 L. Autoclave. 2. Prepare Acrylamide solution as: 6 % Acrylamide 60.0 g Acrylamide 0.25 % Bis-Acrylamide
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