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电泳级丙xī酰胺和双丙xī酰胺在超纯水中配制的溶液。
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文献和实验Shlomit David et al.
Foods (Basel, Switzerland), 9(9) (2020-09-11)
The ribonuclease protection assay (RPA)
. Siliconize the short plate and clean again. Assemble the gel mold (0.4 mm spacers). 22. Combine the following to give a final concentration of 5% acrylamide: 74.5 ml acrylamide solution (final 19:1 acrylamide/bis): 8.85 mls of 40
only; see Steps 40-53)Acrylamide solution for SSCP gels (2X) (e.g., Acrylamide Solution for Mutation Detection, A5934, Sigma) (for SSCP only; see Steps 23-37)MDE Gel Solution is a polyacrylamide-like matrix specifically optimized for SSCP.Acrylamide/bisacrylamide
ELECTROPHORESIS OF DNA IN POLYACRYLAMIDE GELS
all of it has been transferred from the stock bottle. 30% Acrylamide Solution (0.8% bis) 60 g acrylamide 1.6 g bis Heat to dissolve in approximately 100 ml of water. QS to 200 ml with water and filter with Whatman #1 filter paper into a foil
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