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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20˚C
- 英文名:
Pyridoxal phosphate phosphatase
- 库存:
详询
- 供应商:
南京莱富赛
- 规格:
详询
host_species:E.coli
purification:>90% by SDS-PAGE
specificity:Human
other_names:CIN,PLP,PLPP,Chronophin
accession_no:Q96GD0
Gene name:PDXP
calculated_mw:
tag info:His
Immunogen Description:Recombinant protein
formulation:50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole,10%glycerol(PH8.0)
storage:Store at -20C. (Avoid repeated freezing and thawing.)
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
background:Protein serine phosphatase that dephosphorylates 'Ser-3' in cofilin and probably also dephosphorylates phospho-serine residues in DSTN. Regulates cofilin-dependent actin cytoskeleton reorganization. Required for normal progress through mitosis and normal cytokinesis. Does not dephosphorylate phospho-threonines in LIMK1. Does not dephosphorylate peptides containing phospho-tyrosine. Pyridoxal phosphate phosphatase. Has some activity towards pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PMP) and pyridoxine 5'-phosphate (PNP), with a highest activity with PLP followed by PNP.
产品详细信息请点击:Pyridoxal phosphate phosphatase
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文献和实验维生素 B6 的辅酶,在缺乏吡哆醇的培养基上培养的大肠杆菌,其脱羧酶,只有加入吡哆醛和三磷酸腺苷,才能恢复它的活性,从这里所以了解吡哆醛是以与磷酸结合的形态而起作用的,通过从酵母中抽提及合 醇激酶(与吡哆醛激酶同)( 2)是磷酸吡哆醇氧化酶(含有 FMN的黄素酶)。若磷酸吡哆醛本身与丝氨酸、半胱氨酸一起加热,会催化脱氨,但与酶蛋白质和其赖氨酸的ε - 氨基以 Schiff碱基形式结合,作为转氨酶、氨基酸脱羧
Assaying Lipid Phosphate Phosphatase Activities
may be attenuated through the action of lipid phosphate phosphatase enzymes. The levels of lipid phosphate phosphatase activities may be used as a marker of signaling events in the cell. In this chapter we describe enzymatic assays that are routinely used to measure
Purification of acidic phosphatase from mustard seedlings
an artificial substrate that changes its color after hydrolytic removal of the phosphate group: Since the phosphatase is active only at acidic pH values, but p-nitrophenol is colored at basic pH values, we must change the pH following the enzyme
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