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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at -20˚C
- 英文名：
Recombinant Arabidopsis thaliana rRNA 2-O-methyltransferase fibrillarin 1(FIB1)
- 库存：
详询
- 供应商：
南京莱富赛
- 规格：
详询
host_species:E.coli
purification:Greater than 90% as determined by SDS-PAGE.
other_names:Histone-glutamine methyltransferase SKP1-interacting partner 7 rRNA 2'-O-methyltransferase fibrillarin 1 (EC:2.1.1.-)
accession_no:Q9FEF8
calculated_mw:48.8 kDa
tag info:N-terminal 6xHis-SUMO-tagged
Immunogen Description:Expression Region:1-308aa
Sequence Info:Full Length
formulation:Tris-based buffer50% glycerol
storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃,-80℃. The shelf life of lyophilized form is 12 months at -20℃,-80℃.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
background:Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ105me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus
产品详细信息请点击：Recombinant Arabidopsis thaliana rRNA 2-O-methyltransferase fibrillarin 1(FIB1)
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文献和实验1 国内外研究现状 拟南芥（Arabidopsis thaliana ）是一种模式植物，具有基因组小（125 Mbp）、生长周期短等特点，而且基因组测序已经完成（The Arabidopsis Genomic Initiative, 2000）。同时，拟南芥属十字花科（Cruciferae），具有高等植物的一般特点，拟南芥研究中所取得成果很容易用于其它高等植物包括农作物的研究，产生重大的经济效益，特别是十字花科中还有许多重要的经济作物，与人类的生产生活密切相关，因此目前拟南芥的研究越来
inducers. T ren d s P la n t S ci. 3, 352-358. 78. Felenbok, B. (1991) The ethanol utilization regulon of Aspergillus nidulans-the ALCAALCR system as a tool for the expression of recombinant proteins. J. B io tech n o l. 17
【资源】全面归类网址：毕赤酵母表达各种来源蛋白（细菌，真菌，植物，人类等）
mg/L, active333Arabidopsis thaliana NADH:nitrate reductaseI, 18 mg/g[98,99]Arabidopsis thaliana aldehyde oxidaseI, active337Arabidopsis thaliana AtJ2 cochaperone protein250 mg/L,350Arabidopsisthaliana xylosyl transferaseS, 25ug/L490Barley (Hordeum
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