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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Myosin Light Chain 2 Rabbit mAb
- 抗体英文名:
Myosin Light Chain 2 Rabbit mAb
- 应用范围:
WB, IHC, IP
- 宿主:
Recombinant Rabbit
- 适应物种:
Human;Mouse;Rat;Zebrafish
- 抗原来源:
Recombinant Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 保存条件:
Store at -20°C
- 免疫原:
recombinant protein
purification
ProA affinity purified
applications
WB, IHC, IP
appl_detail
WB: 1:1,000-5,000
IHC: 1:50-1:200
other_names
Cardiac myosin light chain-2 antibody
Cardiac ventricular myosin light chain 2 antibody
CMH10 antibody
MLC 2v antibody
MLC-2 antibody
MLC-2v antibody
MLC2 antibody
MLRV_HUMAN antibody
MYL 2 antibody
MYL2 antibody
Myosin light chain 2 regulatory cardiac slow antibody
Myosin light polypeptide 2 regulatory cardiac slow antibody
Myosin regulatory light chain 2 antibody
Myosin regulatory light chain 2 ventricular/cardiac muscle isoform antibody
Regulatory light chain of myosin antibody
RLC of myosin antibody
Slow cardiac myosin regulatory light chain 2 antibody
ventricular/cardiac muscle isoform antibody
calculated_mw
19 kDa
formulation
1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
uniprot
P10916
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文献和实验Myosin Light Chain Preparation
in lyophilizer bottles and freeze in liquid nitrogen.Put on lyophilizer and leave until dry (overnight or over weekend). Day 5 22.Resuspend separate light chain pools 1 and 2 in a small volume of Dialyzing Solution G.Pipette into dialyzing tubing (size #20
Purification of Myosin Light Chain Kinase
RPM (4℃).Filter the supernatant through glass wool and discard the pellet (or save for light chain prep).Save 1 ml of supernatant (4℃)for assay.Add pepstatin and TLCK to final concentrations of 1 μg/ml and 40 Og/ml,respectively. Volume of supernatant
构成肌球蛋白的低分子量(1.5— 2.5万)的亚单位。一分子肌球蛋白有 4个。肌球蛋白有在腺苷三磷酸酶( ATP ase)作用中不可缺少的基本 L链和调节 ATP ase作用的调节 L链。从兔的白肌除去调节 L链( L2 :由 168个氨基酸所成)不影响肌球蛋白的作用。基本 L链( L1 ∶ 190个氨基酸、 L3 ∶ 149个氨基酸)是必要的。兔白肌的肌球蛋白分子,根据 L链的不同而分为 3种,即每分子肌球蛋白中都含有两个 L2 ,其它两个则因种类不同而异,即分 L1
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