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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Myosin light chain kinase Rabbit mAb
- 抗体英文名:
Myosin light chain kinase Rabbit mAb
- 应用范围:
WB, ICC/IF, IHC, FC
- 宿主:
Recombinant Rabbit
- 适应物种:
Human;Mouse;Rat
- 抗原来源:
Recombinant Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 保存条件:
Store at -20°C
- 免疫原:
recombinant protein
purification
ProA affinity purified
applications
WB, ICC/IF, IHC, FC
appl_detail
WB: 1:2,000-1:10,000
IHC: 1:100-1:500
ICC: 1:100-1:500
FC: 1:50-1:100
other_names
deglutamylated form antibody
DKFZp686I10125 antibody
EC 2.7.11.18 antibody
FLJ12216 antibody
Kinase related protein antibody
Kinase-related protein antibody
KRP antibody
MLCK antibody
MLCK1 antibody
MLCK108 antibody
MLCK210 antibody
MSTP083 antibody
MYLK antibody
MYLK_HUMAN antibody
MYLK1 antibody
Myosin light chain kinase antibody
Myosin light polypeptide kinase antibody
OTTHUMP00000180642 antibody
OTTHUMP00000180643 antibody
smMLCK antibody
smooth muscle antibody
Smooth muscle myosin light chain kinase antibody
Telokin antibody
calculated_mw
210 kDa
formulation
1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
uniprot
Q15746
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文献和实验Purification of Myosin Light Chain Kinase
RPM (4℃).Filter the supernatant through glass wool and discard the pellet (or save for light chain prep).Save 1 ml of supernatant (4℃)for assay.Add pepstatin and TLCK to final concentrations of 1 μg/ml and 40 Og/ml,respectively. Volume of supernatant
Myosin Light Chain Preparation
in lyophilizer bottles and freeze in liquid nitrogen.Put on lyophilizer and leave until dry (overnight or over weekend). Day 5 22.Resuspend separate light chain pools 1 and 2 in a small volume of Dialyzing Solution G.Pipette into dialyzing tubing (size #20
构成肌球蛋白的低分子量(1.5— 2.5万)的亚单位。一分子肌球蛋白有 4个。肌球蛋白有在腺苷三磷酸酶( ATP ase)作用中不可缺少的基本 L链和调节 ATP ase作用的调节 L链。从兔的白肌除去调节 L链( L2 :由 168个氨基酸所成)不影响肌球蛋白的作用。基本 L链( L1 ∶ 190个氨基酸、 L3 ∶ 149个氨基酸)是必要的。兔白肌的肌球蛋白分子,根据 L链的不同而分为 3种,即每分子肌球蛋白中都含有两个 L2 ,其它两个则因种类不同而异,即分 L1
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