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- Miltenyi Biotec
- 130-041-301
- 2025年08月22日
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文献和实验Purification of Genomic DNA Using PureLink Silica Columns
实验试剂 Sterile, DNase-free 1.5 ml microcentrifuge tubes for elution Sterile water, pH 7-8.5, if you are using water for elution Wash Buffers (W4) and (W5) Elution Buffer (E1) 实验设备
Affinity chromatography (with HIS-tagged proteins)
and then add 100uL of AC buffer. This is to prevent the contact of protein resin directly with water. At this time the fraction collection tubes can also be labeled. The columns are run standing in eppendorf tubes on ice, the columns are moved to new tubes
the protein sample.MALDI targetMicrocentrifugeMicrocentrifuge tubes (standard and 0.7-mL)METHODFigure 1. Schematic of affinity binding of phosphopeptides to immobilized metal ion affinity columns.Preparation of the Immobilized Metal Ion Affinity Column Insert
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