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- Miltenyi Biotec
- 130-093-129
- 2025年12月19日
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文献和实验Enrichment and Clonal Culture of Hepatic Stem/Progenitor Cells During Mouse Liver Development
livers. FACS clone-sorted single CD45− Ter119− c-kit− CD13+ CD133+ cells (from fetal mid-gestational livers) or CD45− Ter119− c-kit− Sca1− CD13+ CD49f+ CD133+ cells (from adult livers) can form a colony containing both albumin-positive hepatocytes
Assay Cell Enrichment and Detection Kit(PE)Mouse IL-2 Secretion Assay Cell Enrichment and Detection Kit(PE)Mouse IL-4 Secretion Assay Cell Enrichment and Detection Kit(PE) Mouse IL-5 Secretion Assay Cell Enrichment and Detection Kit(PE) Mouse IL-10
. Cell Sorting (Lin-,cKit+,Sca1+): 1. Incubate cells in FcgRII block on ice for 10 min. 2. Stain an aliquot and with PE-IgG2a and FITC-IgG2b isotype antibodies as neg. controls. Incubate remainder of cells with Sca-1 PE, plus c-kit FITC antibodies
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