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文献和实验A number of endogenously biotinylated proteins are found in both cytosol and mitochondria of mammalian cells from many tissues, including liver, spleen, pancreas, kidney, and intestine. Therefore, caution should be taken when using the biotin
Purification and Depletion of RNP Particles by Antisense Affinity Chromatography
Antisense oligonucleotides made of 2′-O -alkyl RNA are useful reagents for the study of the structure and function of ribonucleoprotein (RNP) particles. The nuclease resistant properties of these oligonucleotides, coupled with their ability
in the nucleus in human cell cultures we designed several RNA-biotin based pulldown assays which can be used alone or in combination with other known assays such as ChIP and Flag-tagged pulldown assays. Three protocols are explained
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