Anti-Sca-1 MicroBead Kit(FITC)

AbC Anti-Mouse Bead Kit

实验步骤   1.  Using the AbC™ Anti-Mouse Bead Kit 1)        Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2)        Label

Purification of Lin-, c-kit+, Sca-1+ bone marrow cells for Culture, Flow Cytometry, or Transplantaion

. Cell Sorting (Lin-,cKit+,Sca1+): 1. Incubate cells in FcgRII block on ice for 10 min. 2. Stain an aliquot and with PE-IgG2a and FITC-IgG2b isotype antibodies as neg. controls. Incubate remainder of cells with Sca-1 PE, plus c-kit FITC antibodies

AbC Anti-Rat/Hamster Bead Kit

1 μL   5)      Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC™ capture beads (Component A in the AbC™ Anti-Mouse Bead Kit) to each tube. 6)