FITC anti-mouse Ly-6A/E (Sca-1

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

FC - Quality tested
IF - Reported in the literature

Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity¹, immunohistochemical staining of frozen sections^2,4 and immunofluorescence microscopy⁴, and immunoprecipitation³.

1. Aihara Y, et al. 1986. Eur. J. Immunol. 16:1391. (CMCD)
2. Spangrude GJ, et al. 1988. J. Immunol. 141:3697. (IHC)
3. van de Rijin M, et al. 1989. P. Natl. Acad. Sci. USA 86:4634. (IF)
4. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IHC IF)
5. Rosas M, et al. 2010. J. Leukoc. Biol. 88:169. PubMed

Ly-6A/E is an 18 kD member of the Ly-6 multigene family also known as Sca-1. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses. The E13-161.7 antibody has been reported to be useful for immunohistochemical staining of frozen sections, immunoprecipitation, flow cytometry, and complement-mediated cytotoxicity.