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文献和实验Methods for Human CD8+ T Lymphocyte Proteome Analysis
T lymphocytes, including cytotoxic CD8+ T cells, are important cells involved in immunology, as they can destroy infected or tumor cells. We describe here a detailed protocol starting from CD8+ T lymphocytes isolation for T cell culture followed
Isolation of Untouched Human CD8+ T Cells from Peripheral Blood Mononuclear Cells (PBMC)
-labelled cells during a short incubation. The bead-bound cells are subsequently separated on a magnet and discarded. The remaining, untouched human CD8 T cells can be used for any application. 实验试剂 Magnets: See www.invitrogen.com
Evaluating CD8+ T Cell Responses In Vitro
-release assay. More thorough understanding of CTL biology and killing pathways has more recently resulted in the design of reliable nonradioactive tests to analyze CD8+ T cell responses which are likely to supplant in a close future the 51 Cr-release assay.
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